[Relationship between the Expression of α-syn and Neuronal Apoptosis in Brain Cortex of Acute Alcoholism Rats].
[Relationship between the Expression of α-syn and Neuronal Apoptosis in Brain Cortex of Acute Alcoholism Rats].
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DOI：
10.3969/j.issn.1004-5619.2016.06.002
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
F. Li;Y. Zhang;S. L. Ma
中科院分区：
文献类型：
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作者：
F. Li;Y. Zhang;S. L. Ma
观察急性酒精中毒大鼠脑皮层中α-核蛋白（α-Syn）和神经元凋亡的表达变化的目标，并探索了乙醇对神经元造成的损害的机制。在急性酒精中毒后1小时，3小时，3 h，6 h，6 h和12 h检测到α-syn和caspase-3的酒精饲料。趋势变化进行了分析。在中毒后12小时，在1小时和3小时内仍高于组，caspase-3阳性细胞的数量和大鼠脑皮层的平均光密度逐渐增加。由脑水肿和缺氧引起的SYN可能会参与急性酒精中毒后脑皮质中神经元凋亡的早期阶段。
OBJECTIVES To observe the changes of expression of α-synuclein （α-syn） and neuronal apoptosis in brain cortex of acute alcoholism rats and to explore the mechanism of the damage caused by ethanol to the neurons. METHODS The model of acute alcoholism rat was established by 50% alcohol gavage. The α-syn and caspase-3 were detected by immunohistochemical staining and imaging analysis at 1 h, 3 h, 6 h and 12 h after acute alcoholism. The number of positive cell and mean of optical density were detected and the trend change was analyzed. The variance analysis and t-test were also performed. RESULTS The number of α-syn positive cell and average optical density in brain cortex of acute alcoholism rat increased significantly and peaked at 6 hour with a following slight decrease at 12 h, but still higher than the groups at 1 h and 3 h. Within 12 hours after poisoning, the number of caspase-3 positive cell and average optical density in brain cortex of rats gradually increased. CONCLUSIONS The abnormal aggregation of α-syn caused by brain edema and hypoxia may participate the early stage of neuronal apoptosis in brain cortex after acute alcoholism.