Modulation of hematopoietic and endothelial cell differentiation from mouse embryonic stem cells by different culture conditions.
Modulation of hematopoietic and endothelial cell differentiation from mouse embryonic stem cells by different culture conditions.
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DOI：
10.1182/blood-2004-04-1306
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发表时间：
2005
期刊：
影响因子：
20.3
通讯作者：
W. Zhang;Changwon Park;Elizabeth Arentson;Kyunghee Choi
中科院分区：
文献类型：
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作者：
W. Zhang;Changwon Park;Elizabeth Arentson;Kyunghee Choi
胚胎干细胞在培养过程中可以分化为多种不同的体细胞。为了更好地关联造血和内皮细胞分化的ES细胞在目前可用的协议，我们比较了胎肝激酶-1（Flk-1）-，干细胞白血病（Scl）-和血管内皮钙粘蛋白（VE-钙粘蛋白）-表达细胞中产生的胚状体（EB）和OP 9细胞。我们报告说，Scl和Flk-1的表达动力学在EB和OP 9细胞中是相似的，虽然Flk-1的表达在OP 9细胞上延长。CD 45+和Ter-119+细胞在EB中更有效地发育，而VE-钙粘蛋白+细胞主要在OP 9细胞上发育。细胞分选和再铺板研究表明，Scl+细胞，而不是Flk-1+或VE-钙粘蛋白+细胞，富集原始和永久造血祖细胞。我们的研究表明，最佳的造血和内皮细胞分化发生在EB和OP 9细胞，分别。无论使用哪种培养系统，Scl都是富集原始和永久造血祖细胞的最相关标志物。
Embryonic stem (ES) cells can differentiate into many different somatic cells in culture. To better correlate hematopoietic and endothelial cell differentiation of ES cells in currently available protocols, we compared fetal liver kinase-1 (Flk-1)-, stem cell leukemia (Scl)-, and vascular endothelial-cadherin (VE-cadherin)-expressing cells generated in embryoid bodies (EBs) and on OP9 cells. We report that the kinetics of Scl and Flk-1 expression were similar in EBs and OP9 cells, although Flk-1 expression was extended on OP9 cells. CD45+ and Ter-119+ cells developed more efficiently in EBs, whereas VE-cadherin+ cells developed largely on OP9 cells. Cell sorting and replating studies showed that Scl+ cells, not Flk-1+ or VE-cadherin+ cells, were enriched for primitive and definitive hematopoietic progenitors. Our studies indicate that optimal hematopoietic and endothelial cell differentiation occur in EBs and on OP9 cells, respectively. Regardless of the culture systems used, Scl is the most relevant marker for enriching primitive and definitive hematopoietic progenitors.