Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice
Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice
复制标题
糖精多糖在短期高脂饮食喂养小鼠肠道微生物群调节中的组成-活性关系
DOI：
10.1021/acs.jafc.1c04490
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发表时间：
2021-09-09
影响因子：
6.1
通讯作者：
Wang, Hong
中科院分区：
文献类型：
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作者：
Ke, Songze;Yu, Yanlei;Wang, Hong
糖精多糖可调节肠道菌群组成；然而，成分与活性的关系尚不明确，限制了其应用。在本研究中，我们研究了8种不同的粳米多糖组分在高脂饲料（HFD）喂养小鼠第2天和第14天对肠道微生物群的影响。结果显示，2天的HFD显著改变了肠道菌群组成，另外12天的HFD进一步加强了HFD组的肠道菌群失调。LjA-1和LjA-3能部分缓解肠道菌群组成失调，显著改变肠道菌群功能。多元线性回归分析表明，硫酸盐含量和分子量分布是影响肠道优势菌属的主要因素。我们的研究结果表明，在第2天，HFD可能会扰乱肠道微生物群的稳态，并为多糖在调节肠道微生物群中的定量组成-活性关系提供了见解。
Saccharina japonica polysaccharide could modulate gut microbiota composition; however, the composition-activity relationship remains unclear, thus restricting its application. In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice. The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group. LjA-1 and LjA-3 could partially alleviate the dysbiosis of gut microbiota composition and significantly alter gut microbiota function. Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera. Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.