Lactoferrin modified graphene oxide iron oxide nanocomposite for glioma-targeted drug delivery
Lactoferrin modified graphene oxide iron oxide nanocomposite for glioma-targeted drug delivery
复制标题
乳铁蛋白修饰氧化石墨烯氧化铁纳米复合材料用于神经胶质瘤靶向药物输送
DOI：
10.1016/j.msec.2017.03.309
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发表时间：
2017-08-01
影响因子：
7.9
通讯作者：
Shen, Yu-Xian
中科院分区：
文献类型：
--
作者：
Song, Meng-Meng;Xu, Huai-Liang;Shen, Yu-Xian
Targeting delivery of drugs in a specific manner represents a potential powerful technology in gliomas. Herein, we prepared a multifunctional targeted delivery system based on graphene oxide (GO) that contains a molecular bio-targeting ligand and superparamagnetic iron oxide nanoparticles on the surface of GO for magnetic targeting. Superparamagnetic Fe3O4 nanoparticles was loaded on the surface of GO via chemical precipitation method to form GO@Fe3O4 nanocomposites. Lactoferrin (Lf), an iron -transporting serum glycoprotein that binds to receptors overexpressed at the surface of glioma cells and vascular endothelial cell of the blood brain barrier, was chosen as the targeted ligand to construct the targeted delivery systemlf@GO@Fe3O4 through EDC/NHS chemistry. With the confirmation of TEM, DLS and VSM, the resulting Lf@GO@Fe3O4 had a size distribution of 200-1000 nm and exhibited a superparamagnetic behavior. The nano delivery system had a high loading capacity and exhibited a pH -dependent release behavior. Compared with free DOX and DOX@GO@Fe3O4, Lf@GO@Fe3O4@DOX displayed greater intracellular delivery efficiency and stronger cytotoxicity against C6 glioma cells. The results demonstrated the potential utility of Lf conjugated GO@Fe3O4 nanocomposites for therapeutic application in the treatment of gliomas. (C) 2017 Published by Elsevier B.V.
