Effect of phosphorus levels on the protein profiles of secreted protein and root surface protein of rice
Effect of phosphorus levels on the protein profiles of secreted protein and root surface protein of rice
复制标题
磷水平对水稻分泌蛋白和根表面蛋白蛋白质谱的影响
DOI：
10.1021/pr400614n
复制
发表时间：
2013
影响因子：
4.4
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Shinano;T.;Yoshimura;T.;Watanabe;T.;Unno;Y.;Osaki;M.;Nanjo;Y. and Komatsu;S.
植物根是从土壤中吸收水分和养分的复杂器官。根在保护植物免受土壤病原体攻击方面也发挥着重要作用，并对某些土壤微生物发挥有益作用。植物来源的根际蛋白（例如根分泌蛋白和根表面结合蛋白）被认为在根际发展相互关系中发挥重要作用。在根际，植物根与周围环境相遇，有人认为根分泌蛋白和根表面结合蛋白是重要因素。此外，尚不清楚植物的生理状态如何影响这些蛋白质的特征。在本研究中，水稻植株在无菌条件下生长，有或没有磷营养，从根浴液中获得蛋白质（称为根分泌蛋白），并使用 0.2 M CaCl2 溶液获得蛋白质（称为根表面结合蛋白）。根浴液中鉴定出的蛋白质总数为 458 个，根表面结合蛋白的数量为 256 个。在两个组分中都观察到了一半以上的蛋白质。大多数蛋白质被分类为具有信号肽或不具有膜转运螺旋位点。功能分类表明大多数蛋白质似乎具有分泌途径并参与防御/疾病相关功能。这些特征似乎是根际蛋白质所独有的，后者可能是植物击败土壤中病原体的策略的一部分。低磷处理显着增加了根分泌蛋白中发病机制相关蛋白的数量，而根表面结合蛋白的变化较小。结果表明，根部主动且选择性地将蛋白质分泌到根际。
Plant roots are complicated organs that absorb water and nutrients from the soil. Roots also play an essential role in protecting plants from attack by soil pathogens and develop a beneficial role with some soil microorganisms. Plant-derived rhizosphere proteins (e.g., root secretory proteins and root surface binding proteins) are considered to play important roles in developing mutual relationships in the rhizosphere. In the rhizosphere, where plant roots meet the surrounding environment, it has been suggested that root secretory protein and root surface binding protein are important factors. Furthermore, it is not known how the physiological status of the plant affects the profile of these proteins. In this study, rice plants were grown aseptically, with or without phosphorus nutrition, and proteins were obtained from root bathing solution (designated as root secretory proteins) and obtained using 0.2 M CaCl2solution (designated as root surface binding proteins). The total number of identified proteins in the root bathing solution was 458, and the number of root surface binding proteins was 256. More than half of the proteins were observed in both fractions. Most of the proteins were categorized as either having signal peptides or no membrane transport helix sites. The functional categorization suggested that most of the proteins seemed to have secretory pathways and were involved in defense/disease-related functions. These characteristics seem to be unique to rhizosphere proteins, and the latter might be part of the plants strategy to defeat pathogens in the soil. The low phosphorus treatment significantly increased the number of pathogenesis-related proteins in the root secretory proteins, whereas the change was small in the case of the root surface binding proteins. The results suggested that the roots are actively and selectively secreting protein into the rhizosphere.