SEQUENCE COMPLEXITY OF NUCLEAR AND POLYSOMAL RNA IN LEAVES OF TOBACCO PLANT
SEQUENCE COMPLEXITY OF NUCLEAR AND POLYSOMAL RNA IN LEAVES OF TOBACCO PLANT
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DOI：
10.1016/0092-8674(78)90307-0
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
TIMBERLAKE, WE
中科院分区：
文献类型：
--
作者：
GOLDBERG, RB;HOSCHEK, G;TIMBERLAKE, WE
The first measurements are reported of the sequence complexity of nuclear and polysomal RNA contained within the cells of a higher plant. Polysomal RNA from tobacco leaves was prepared by a procedure which minimized contamination with nuclear RNA. Hybridization of 3H-cDNA complementary to polysomal poly(A) RNA with an excess of tobacco DNA indicated that > 95% of the poly(A) mRNA was transcribed from single-copy sequences. RNA excess hybridization reactions with polysomal poly(A) RNA and 3H-cDNA revealed the presence of 3 abundance classes in the poly(A) mRNA. The best least-squares solution indicated that these classes comprise 9, 52 and 39% of the poly(A) mRNA and contain sequences present an average of 4500, 340 and 17 times per cell. Hybridization reactions containing an excess of nuclear or total polysomal RNA and 3H-single-copy DNA indicated the complexity of these RNA populations to be 1.19 .times. 108 nucleotides (nuclear) and 3.33 .times. 107 nucleotides (polysomal). Thus only 28% of the nuclear RNA sequence diversity (27,000 average-sized mRNA sequences) is represented in leaf polysomes. Apparently there is a general similarity in the basic transcriptional processes of metaphytan and metazoan cells.
