Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins
Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins
复制标题
DOI：
10.1016/j.febslet.2007.06.022
复制
发表时间：
2007-07-10
期刊：
影响因子：
3.5
通讯作者：
Dalmay, Tamas
中科院分区：
文献类型：
--
作者：
Ho, Thien;Wang, Hui;Dalmay, Tamas
从芜菁花叶病毒（Turnip mosaic virus，TuMV）侵染的芥菜叶片中提取小分子干扰RNA（Small interfering RNA，si RNA），对其进行克隆和序列分析。TuMV siRNA群体主要由主要源自相同siRNA热点的21和22-nt长的种类占主导地位，表明植物Dicer样（DCL）酶之间的操作相似性。TuMV siRNA相对于病毒基因组观察到稳健的GC偏倚，表明DCL更可能靶向富含GC的区域。此外，双子叶植物微（mi）RNA显示出比它们的DCL 1底物RNA更高的GC%，暗示GC偏好可能是古老的，因此对于RNAi技术可能是重要的。(C)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant Dicer-like (DCL) enzymes. Robust GC bias was observed for TuMV siRNAs versus the virus genome, indicating that DCL was more likely to target GC-rich regions. Furthermore, dicot micro-(mi)RNAs displayed higher GC% than their DCL1 substrate RNAs, implicating that the GC bias may be ancient, therefore may be important for the RNAi technology. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.