A vacuole-like compartment concentrates a disordered calcium phase in a key coccolithophorid alga.
A vacuole-like compartment concentrates a disordered calcium phase in a key coccolithophorid alga.
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DOI：
10.1038/ncomms11228
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发表时间：
2016-04-14
影响因子：
16.6
通讯作者：
Scheffel A
中科院分区：
文献类型：
--
作者：
Sviben S;Gal A;Hood MA;Bertinetti L;Politi Y;Bennet M;Krishnamoorthy P;Schertel A;Wirth R;Sorrentino A;Pereiro E;Faivre D;Scheffel A
颗石是在称为颗石藻的单细胞海藻细胞内产生的方解石颗粒。它们是海底碳酸盐的丰富成分，并且化石球石中钙与其他元素的化学计量被广泛用于推断过去的环境条件。在这里，我们使用最先进的纳米级成像和光谱学研究了优势球石藻 Emiliania Huxleyi 的冷冻保存细胞。我们确定了一个与产生石炭酸的隔室不同的隔室，其中充满了高浓度的无序形式的钙。与钙共存的是高浓度的磷和少量的其他阳离子。储存在该储库中的钙量似乎是动态的，并且在某个阶段，该隔室与产生石炭酸的囊泡直接接触，这表明在石炭酸形成中发挥着积极作用。我们的研究结果提供了对这种重要钙化生物体中钙积累的见解。 颗石藻是单细胞海洋藻类，在细胞内产生方解石颗粒。在这里，作者研究了占优势的球石藻赫胥黎石藻的细胞，并确定了一个充满高浓度无序钙形式的细胞内隔室。
Coccoliths are calcitic particles produced inside the cells of unicellular marine algae known as coccolithophores. They are abundant components of sea-floor carbonates, and the stoichiometry of calcium to other elements in fossil coccoliths is widely used to infer past environmental conditions. Here we study cryo-preserved cells of the dominant coccolithophore Emiliania huxleyi using state-of-the-art nanoscale imaging and spectroscopy. We identify a compartment, distinct from the coccolith-producing compartment, filled with high concentrations of a disordered form of calcium. Co-localized with calcium are high concentrations of phosphorus and minor concentrations of other cations. The amounts of calcium stored in this reservoir seem to be dynamic and at a certain stage the compartment is in direct contact with the coccolith-producing vesicle, suggesting an active role in coccolith formation. Our findings provide insights into calcium accumulation in this important calcifying organism. Coccolithophores are unicellular marine algae that produce calcitic particles inside their cells. Here the authors study cells of the dominant coccolithophore Emiliania huxleyi and identify an intracellular compartment that is filled with high concentrations of a disordered form of calcium.