LENGTH HETEROGENEITY OF A CONSERVED DISPLACEMENT-LOOP SEQUENCE IN HUMAN MITOCHONDRIAL-DNA
LENGTH HETEROGENEITY OF A CONSERVED DISPLACEMENT-LOOP SEQUENCE IN HUMAN MITOCHONDRIAL-DNA
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DOI：
10.1093/nar/13.22.8093
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
CLAYTON, DA
中科院分区：
文献类型：
--
作者：
HAUSWIRTH, WW;CLAYTON, DA
来自人组织培养细胞的线粒体DNA含有位于先前鉴定的进化保守区域（称为CSB-2）内的异质序列。CSB-2位于重链线粒体DNA合成的起点附近，并且是人线粒体DNA每条链的主要转录启动子。克隆的线粒体DNA的核苷酸序列分析和来自细胞线粒体DNA的适当小片段的电泳分析表明，变异性限于长度范围为6至12个残基的均聚物序列。证明最常见的多晶型物将支持准确的转录起始。
Mitochondrial DNA from human tissue culture cells contains heterogeneous sequences located within a previously identified, evolutionally conserved region termed CSB-2. CSB-2 is located near the origin of heavy-strand mitochondrial DNA synthesis and the major transcriptional promoters for each strand of human mitochondrial DNA. Nucleotide sequence analysis of cloned mitochondrial DNA and electrophoretic analysis of appropriate small fragments from cellular mitochondrial DNA show that the variability is limited to a homopolymer sequence which can range in length from 6 to 12 residues.Invitrotranscriptional analyses, using several of these cloned length polymorphs as templates and partially purified human mitochondrial RNA polymerase, demonstrate that the most common polymorphs will support accurate transcriptional initiation.