Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification.
Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification.
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通过逆转录回路介导的等温扩增来快速检测猫科动病毒。
DOI：
10.1292/jvms.15-0239
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Miyazawa T
中科院分区：
文献类型：
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作者：
Koide R;Sakaguchi S;Ogawa M;Miyazawa T
猫麻疹病毒(FmoPV)是一种在家猫中出现的新病毒，被认为是猫慢性肾功能衰竭的原因之一。在本研究中，我们建立了一种检测FmoPV的逆转录环介导等温扩增(RT-LAMP)方法。结果表明，该方法对原始样品的检出限为10~50%组织培养感染量(TCID50)/ml，灵敏度为0.12 TCID50/1次RT-LAMP反应。我们还在感染FmoPV的猫的临床尿样中检测到FmoPV。FmoPV RT-LAMP检测方法快速、简便、特异性高，是一种可靠的FmoPV检测方法。
Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID50)/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID50 per one RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infected with FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detection of FmoPV, and thus, it would be a reliable detection method for FmoPV.