PTOV1 is associated with UCH-L1 and in response to estrogen stimuli during the mouse oocyte development
PTOV1 is associated with UCH-L1 and in response to estrogen stimuli during the mouse oocyte development
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PTOV1 与 UCH-L1 相关，并在小鼠卵母细胞发育过程中对雌激素刺激做出反应
DOI：
10.1007/s00418-011-0825-z
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发表时间：
2011-08-01
影响因子：
2.3
通讯作者：
Sun, Zhao-Gui
中科院分区：
文献类型：
--
作者：
Yao, Yu-Wei;Shi, Yan;Sun, Zhao-Gui
为了研究泛素羧基末端水解酶 L1 (UCH-L1) 参与卵母细胞成熟的生物学意义，我们筛选了小鼠卵巢中与 UCH-L1 结合的蛋白，发现前列腺肿瘤过表达 1 (PTOV1) 蛋白能够与 UCH-L1 结合。 PTOV1在前列腺癌中高表达，被认为是前列腺癌发生和进展的潜在标志物。据报道，PTOV1在细胞周期调节中发挥重要作用，但其在哺乳动物卵母细胞发育和减数分裂中的作用仍不清楚。本文发现，从青春期前到成年期，小鼠卵巢中PTOV1的表达水平逐渐升高。我们通过免疫组化发现，在青春期前，PTOV1在卵母细胞的细胞质和细胞核中都有分布，但在正常成年小鼠卵母细胞中，它不仅集中在细胞核中，而且集中在质膜上，尽管在一些形状异常的卵母细胞中，PTOV1没有表现出典型的分布模式。然而，在颗粒细胞中，它被发现在所有选定的年龄都位于细胞质中。在出生后小鼠卵巢（28 天）中，雌二醇治疗诱导卵母细胞中 PTOV1 的成人特异性分布模式。此外，UCH-L1被证明与CDK1相关，CDK1参与细胞周期和卵母细胞成熟的调节。因此，我们认为PTOV1的分布变化具有年龄依赖性，并且对小鼠卵母细胞的发育和成熟具有重要意义。此外，PTOV1与小鼠卵母细胞中的UCH-L1相关的发现支持了UCH-L1参与卵母细胞发育和成熟的解释，特别是在雌激素的调节下。
To investigate the biological significance of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) involvement in oocyte maturation, we screened for proteins that bound to UCH-L1 in mouse ovaries, and we found that the prostate tumor overexpressed-1 (PTOV1) protein was able to bind to UCH-L1. PTOV1 is highly expressed in prostate cancers and considered as a potential marker for carcinogenesis and the progress of prostate cancer. It was reported that PTOV1 plays an important role in cell cycle regulation, but its role in mammalian oocyte development and meiosis is still unclear. In this paper, it was found that the expression levels of PTOV1 in mouse ovaries progressively increased from prepubescence to adulthood. And we found by immunohistochemistry that PTOV1 spreaded in both the cytoplasm and nuclei of oocytes during prepuberty, but in normal adult mouse oocytes, it concentrated not only in nuclei but also on the plasma membrane, though in some oocytes with abnormal shapes, PTOV1 did not display the typical distribution patterns. In granulosa cells, however, it was found to locate in the cytoplasm at all the selected ages. In postnatal mouse ovaries (28 days), estradiol treatment induced the adult-specific distribution pattern of PTOV1 in oocytes. In addition, UCH-L1 was shown to be associated with CDK1, which participated in the regulation of cell cycle and oocyte maturation. Therefore, we propose that the distribution changes of PTOV1 are age-dependent, and significant for mouse oocyte development and maturation. Moreover, the discovery that PTOV1 is associated with UCH-L1 in mouse oocytes supports the explanations for that UCH-L1 is involved in oocyte development and maturation, especially under the regulation of estrogen.