Simple oral mucosal epithelial transplantation in a rabbit model
Simple oral mucosal epithelial transplantation in a rabbit model
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DOI：
10.1038/s41598-019-54571-7
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发表时间：
2019-12-02
影响因子：
4.6
通讯作者：
Yamagami, Satoru
中科院分区：
文献类型：
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作者：
Inamochi, Aya;Tomioka, Akiko;Yamagami, Satoru
本研究建立兔自体单纯性口腔黏膜上皮移植治疗角膜缘干细胞缺乏症（LSCD）的模型。在SOMET组和Control组创建LSCD。在SOMET组中，从颊区收获的口腔黏膜经疾病处理，切成小块，放置在暴露的角膜基质上，不使用移植缝合线、羊膜和/或胶水。两组均放置软性隐形眼镜，并行眼痂修补术。采用裂隙灯显微镜观察两组术后角膜新生血管形成情况及荧光素染色评分。术后2周，切除双眼，进行CK3、CK13、CK15和p63的免疫组化染色。在SOMET组中，口腔黏膜移植导致LSCD完全恢复，术后2周，新生血管评分低，荧光素染色评分低，基质上检测到分层的K3/ k13阳性细胞。相比之下，对照组的角膜上皮缺陷在2周后仍然存在。SOMET在兔LSCD模型中实现了角膜表面的再上皮化。这是一种简单的技术，不需要培养，可能是双侧LSCD眼表重建的一个有前途的选择。
y This study investigated a rabbit model of autologous simple oral mucosal epithelium transplantation (SOMET) for limbal stem cell deficiency (LSCD). LSCD was created in the SOMET group and the Control group. In the SOMET group, oral mucosa harvested from the buccal region was treated with dispase, cut into small pieces, and placed on the exposed corneal stroma without using graft sutures, amniotic membrane, and/or glue. A soft contact lens was positioned and tarsorrhaphy was performed in both groups. Postoperative corneal neovascularization and fluorescein staining scores were evaluated by slit lamp microscopy in both groups. At 2 weeks postoperatively, eyes were excised and subjected to immunohistochemical staining for CK3, CK13, CK15, and p63. In the SOMET group, transplantation of oral mucosa led to complete recovery of LSCD, as indicated by low neovascularization scores, low fluorescein staining scores, and detection of stratified K3/K13-positive cells on the stroma at 2 weeks after surgery. In contrast, corneal epithelial defects persisted in the Control group at 2 weeks. SOMET achieved re-epithelialization of the corneal surface in this rabbit LSCD model. It is a simple technique that does not require culture and could be a promising option for ocular surface reconstruction in bilateral LSCD.