Development of a dispersive liquid-liquid microextraction technique for the analysis of citrinin, alternariol and alternariol monomethyl ether in fruit juices
Development of a dispersive liquid-liquid microextraction technique for the analysis of citrinin, alternariol and alternariol monomethyl ether in fruit juices
复制标题
开发分散液-液微萃取技术用于分析果汁中的柑橘素、交链格醇和交链格醇单甲醚
DOI：
10.1039/c6ay02456d
复制
发表时间：
2016
影响因子：
3.1
通讯作者：
Liu Chenglan
中科院分区：
文献类型：
--
作者：
Ruan Chunqiang;Diao Xue;Zhang He;Zhang Liyang;Liu Chenglan
建立了分散液液微萃取（DLLME）-高效液相色谱荧光检测法测定果汁中桔霉素、交链孢酚和交链孢酚甲醚的新方法。在DLLME过程中的有效参数，包括氯化钠的百分比，pH值，和体积的提取和分散溶剂，使用响应面法的基础上的中心组合设计进行了优化。在最佳条件下，果汁中3种霉菌毒素的平均回收率在80.1-93.4%之间，相对标准偏差小于5.7%。检测限为0.022-0.203 ng mL−1。与以往方法的比较表明，该方法是一种准确，快速，可靠的样品前处理方法，提供了很好的富集因子和检测限的提取和测定果汁中的桔霉素，交链孢酚和交链孢酚单甲醚。
A new method based on dispersive liquid–liquid microextraction (DLLME) combined with high-performance liquid chromatography with fluorescence detection has been established to detect citrinin, alternariol and alternariol monomethyl ether in fruit juices. The effective parameters in the DLLME process, including sodium chloride percentage, pH, and volumes of extraction and dispersive solvents, were optimized using the response surface methodology based on a central composite design. Under the optimum conditions, mean recoveries of the three mycotoxins from fruit juices were in the range of 80.1–93.4% with relative standard deviations lower than 5.7%. The detection limits were in the range of 0.022–0.203 ng mL−1. A comparison of this method with previous methods demonstrated that the proposed method is an accurate, rapid and reliable sample-pretreatment method that gives very good enrichment factors and detection limits for extracting and determining citrinin, alternariol and alternariol monomethyl ether in fruit juices.