Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
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DOI：
10.1038/bjc.1993.12
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发表时间：
1993-01
影响因子：
8.8
通讯作者：
Makita A
中科院分区：
文献类型：
--
作者：
Kobayashi T;Honke K;Kamio K;Sakakibara N;Gasa S;Miyao N;Tsukamoto T;Ishizuka I;Miyazaki T;Makita A
从人肾细胞癌建立的细胞系 (SMKT-R3) 的特征在于存在硫脂和糖脂磺基转移酶。发现 SMKT-R3 细胞中酸性糖脂部分的很大一部分是硫脂。这些发现通过 35S-硫酸盐代谢标记得到证实。使用针对硫脂的单克隆抗体通过细胞荧光测定法确定，这些硫脂在 SMKT-R3 细胞表面表达。此外，与其他细胞系相比，SMKT-R3 细胞中糖脂磺基转移酶的活性水平明显较高。这些结果表明，肾细胞癌组织中硫脂的合成增加(Sakakibara等人，1989.Cancer Res.，49, 335-339)是由于肾癌细胞本身的磺基转移酶活性升高所致。
A cell line (SMKT-R3) established from human renal cell carcinoma was characterized for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells. These findings were confirmed by metabolic labelling with 35S-sulfate. These sulfolipids were expressed at the surface of SMKT-R3 cells as ascertained by cytofluorometry using a monoclonal antibody directed to sulfolipids. Furthermore, markedly high activity levels of glycolipid sulfotransferases were observed in SMKT-R3 cells compared with other cell lines. These results suggest that the increased synthesis of sulfolipids in renal cell carcinoma tissue (Sakakibara et al., 1989. Cancer Res., 49, 335-339) is due to the elevation of the sulfotransferase activities of renal carcinoma cells themselves.