Misidentifications of Echinochloa crus‐galli var. formosensis as Echinochloa oryzicola as a result of similar cpDNA sequences
Misidentifications of Echinochloa crus‐galli var. formosensis as Echinochloa oryzicola as a result of similar cpDNA sequences
复制标题
DOI：
10.1111/wbm.12185
复制
发表时间：
2019-09
影响因子：
1.4
通讯作者：
K. Yasuda;Y. Nakayama
中科院分区：
文献类型：
--
作者：
K. Yasuda;Y. Nakayama
稗草Echinochloa australgalli（L.）博夫变种台湾杂草（2n = 6x = 54，AABBCC基因组）和稻穗杂草（Echinochloa oryzicola（Vasinger）Vasinger（2n = 4x = 36，AABB））是东亚和东南亚的主要水稻杂草。e. oryzicola通常被认为是E.没什么L.，其中包括E.加利河基于cpDNA序列的台湾矮牵牛。因此，使用聚合酶链反应-限制性片段长度多态性分析的cpDNA的分子表征已被提出作为区分两个物种的可靠方法。在这项研究中，我们报告了四个E。加利河来自冲绳、长崎、静冈和东京的福尔摩沙与E. oryzicola，并被误鉴定为E.利用分子生物学方法研究稻瘟病菌。此外，我们的研究结果表明，这些材料可能继承了他们的叶绿体基因组从E。在多倍化过程中，它们不是来自一个无名的二倍体物种。我们的发现为E.没什么L.提示单纯用cpDNA分子鉴定方法不能区分E.加利河formosensis和E.稻
Echinochloa crus‐galli (L.) Beauv. var. formosensis Ohwi (2n = 6x = 54, AABBCC genomes) and Echinochloa oryzicola (Vasinger) Vasinger (2n = 4x = 36, AABB) are major paddy weeds in East and Southeast Asia. E. oryzicola has been generally considered to be a paternal genome donor of E. crus‐galli s. l., which includes E. crus‐galli var. formosensis based on cpDNA sequences. Thus, molecular characterization using polymerase chain reaction‐restriction fragment length polymorphism analysis of cpDNA has been proposed as a reliable method for discriminating between the two species. In this study, we report that four accessions of E. crus‐galli var. formosensis from Okinawa, Nagasaki, Shizuoka and Tokyo had similar cpDNA sequences to E. oryzicola and had been misidentified as E. oryzicola using molecular methods. In addition, our results demonstrated that these accessions likely inherited their chloroplast genomes from E. oryzicola and not from an anonymous diploid species during polyploidization. Our findings provide new insights into the evolution of E. crus‐galli s. l. and suggest that identification using the cpDNA molecular method alone is not an appropriate approach to differentiate E. crus‐galli var. formosensis and E. oryzicola.