Reparability of DNA double-strand breaks and radiation sensitivity in five mammalian cell lines.
Reparability of DNA double-strand breaks and radiation sensitivity in five mammalian cell lines.
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五种哺乳动物细胞系 DNA 双链断裂的可修复性和辐射敏感性。
DOI：
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发表时间：
1991
影响因子：
2.6
通讯作者：
I. Kaneko
中科院分区：
文献类型：
--
作者：
K. Eguchi;T. Kosaka;K. Sato;I. Kaneko
我们研究了γ射线诱导的DNA双链断裂（dsb）和细胞致死率之间的关系，在5个哺乳动物细胞系不同的放射敏感性; HA-1，HMV-I，L5178 Y，M10和LX 830。这些细胞分别来源于中国仓鼠、人黑素瘤和小鼠淋巴瘤（亲本细胞及其放射敏感突变体）。HA-1细胞对辐射最不敏感，LX 830细胞对辐射最敏感。尽管HA-1的γ射线诱导dsb与其他曲线显著不同（HMV-1的p小于0.05，L5178 Y和M10的p小于0.01），但其他四个品系的曲线彼此相似。此外，最耐辐射的细胞系，HA-1，表现出最高的dsb诱导五个细胞系。因此，这五个品系的辐射敏感性与dsb的诱导之间没有相关性。另一方面，修复孵育后残留的dsb（不可修复dsb）确实彼此不同。当不可修复dsb的相对数量对辐射剂量作图时，所有细胞系的剂量-反应曲线都是凹形的，并且M10和LX 830的曲线斜率比其他细胞系的曲线斜率更陡。这些曲线是生存曲线的镜像。结果表明，细胞杀伤的辐射抗性与细胞修复dsb的能力之间存在相关性。
We examined the relationship between gamma-ray-induced DNA double-strand breaks (dsb) and cell lethality in five mammalian cell lines differing in radiosensitivity; HA-1, HMV-I, L5178Y, M10 and LX830. These cells were derived from Chinese hamster, human melanoma, and mouse lymphoma (parent and its radiosensitive mutants), respectively. HA-1 cells were the most radioresistant and LX830 cells were the most radiosensitive among these five lines. Although the induction of dsb by gamma-rays for HA-1 was significantly different from other curves (p less than 0.05 for HMV-I and p less than 0.01 for L5178Y and M10), those for the other four lines were similar to one another. In addition, the most radioresistant cell line, HA-1, showed the highest dsb induction among five cell lines. Therefore, there is no correlation between radiosensitivity and the induction of dsb in these five lines. On the other hand, residual dsb after repair incubation (non-reparable dsb) do differ from each other. When the relative number of non-reparable dsb was plotted against the radiation dose, the dose-response curves for all the cell lines were concave, and the slopes of curves for M10 and LX830 were steeper than those for other cell lines. These curves are a mirror-image of the survival curves. The results suggest that there is a correlation between the radio-resistance in terms of cell killing and the capacity of cells to repair dsb.
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DOI：
10.1016/0360-3016(88)90125-3
发表时间：
1988
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
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DOI：
10.1073/pnas.84.21.7562
发表时间：
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影响因子：
11.1
作者：
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影响因子：
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影响因子：
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作者：
Okayasu,R;Iliakis,G
通讯作者：
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影响因子：
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