1H NMR (500 MHz) identification of aromatic residues of gene 32 protein involved in DNA binding by use of protein containing perdeuterated aromatic residues and by site-directed mutagenesis.
1H NMR (500 MHz) identification of aromatic residues of gene 32 protein involved in DNA binding by use of protein containing perdeuterated aromatic residues and by site-directed mutagenesis.
复制标题
通过使用含有全氘代芳香族残基的蛋白质和定点诱变，对参与 DNA 结合的基因 32 蛋白质的芳香族残基进行 1 H NMR (500 MHz) 鉴定。
DOI：
10.1021/bi00360a029
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Coleman,JE
中科院分区：
文献类型：
--
作者：
Prigodich,RV;Shamoo,Y;Williams,KR;Chase,JW;Konigsberg,WH;Coleman,JE
材料和方法Gene 32 蛋白质。同源基因 32 蛋白的制备如前所述（Bittner 等人，1979）。酪氨酸、苯丙氨酸或色氨酸营养缺陷型大肠杆菌宿主由 Barbara Bachman 博士从美国培养物保藏中心友情提供。这
Materials and MethodsGene 32 Proteins. Homogeneous gene 32 protein was prepared as previously described (Bittner et al., 1979). Es-cherichia coli hosts auxotrophic for the amino acids tyrosine, phenylalanine, or tryptophan were kindly supplied from the American Culture Collection by Dr. Barbara Bachman. The