Early BrdU-responsive genes constitute a novel class of senescence-associated genes in human cells
Early BrdU-responsive genes constitute a novel class of senescence-associated genes in human cells
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DOI：
10.1016/j.yexcr.2004.10.036
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发表时间：
2005-04-01
影响因子：
3.7
通讯作者：
Ayusawa, D
中科院分区：
文献类型：
--
作者：
Minagawa, S;Nakabayashi, K;Ayusawa, D
通过DNA芯片和Northern印迹分析，我们在永生成纤维细胞系SUSM-1中发现了对5-溴脱氧尿苷(BrdU)有直接反应的基因。在加入BrdU后的36小时内，至少有29个基因被发现改变基因表达超过对照组的两倍或更少。它们在加入BrdU后表现出几种不同的表达模式，大多数在12h内发生了显著的变化。当比较正常的人成纤维细胞SUSM-1、HeLa和TIG-7时，有19个基因在加入BrdU后表现出相似的表现。此外，14个基因，其中9个是关于衰老的新基因，在衰老的TIG-7细胞中表现类似。这些基因似乎对细胞增殖或细胞周期进程没有作用。这些结果表明，早期BrdU反应基因代表了细胞衰老的早期迹象，可以作为其新的生物标志物。(C)2004 Elsevier Inc.保留所有权利。
We identified genes that immediately respond to 5-bromodeoxyuridine (BrdU) in SUSM-1, an immortal fibroblastic line, with DNA microarray and Northern blot analysis. At least 29 genes were found to alter gene expression greater than twice more or less than controls within 36 h after addition of BrdU. They took several different expression patterns upon addition of BrdU, and the majority showed a significant alteration within 12 h. When compared among SUSM-1, HeLa, and TIG-7 normal human fibroblasts, 19 genes behaved similarly upon addition of BrdU. In addition, 14 genes, 9 of which are novel as regards senescence, behaved similarly in senescent TIG-7 cells. The genes do not seem to have a role in proliferation or cell cycle progression. These results suggest that the early BrdU-responsive genes represent early signs of cellular senescence and can be its new biomarkers. (c) 2004 Elsevier Inc. All rights reserved.