HPLC retention behavior on hydride-based stationary phases.
HPLC retention behavior on hydride-based stationary phases.
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DOI：
10.1002/jssc.200600414
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发表时间：
2007-03
影响因子：
3.1
通讯作者：
J. Pesek;M. Matyska;S. Larrabee
中科院分区：
文献类型：
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作者：
J. Pesek;M. Matyska;S. Larrabee
两个固定相连接到硅氢化物表面，胆固醇和双齿C18，研究了一些药学上相关的化合物，以说明这些键合材料的各种保留机制是可能的。根据log P（辛醇/水分配系数）和pKa值，供试品溶质的范围从亲水性到疏水性。移动的相由酸化（甲酸和高氯酸）水/甲醇或水/ACN混合物组成。特别令人感兴趣的是高有机含量的移动的相组合物，其中如果粘结材料在含水正相（ANP）模式下操作，则保留将增加。使用保留因子（k）与移动的相组成的曲线来阐明保留机制。提出了一些基于RP、ANP或双重保留机制保留溶质的实例。基于二氧化硅的固定相还可以将化合物保留在有机正相中。
Two stationary phases attached to a silica hydride surface, cholesterol and bidentate C18, are investigated with a number of pharmaceutically related compounds in order to illustrate the various retention mechanisms that are possible for these bonded materials. The test solutes range from hydrophilic to hydrophobic based on log P (octanol/water partition coefficient) and pKa values. The mobile phases consist of acidified (formic and perchloric acid) water/methanol or water/ACN mixtures. Of particular interest are the high organic content mobile phase compositions where the retention would increase if the bonded material was operating in the aqueous normal phase (ANP) mode. Plots of retention factor (k) versus mobile phase composition are used to elucidate the retention mechanism. A number of examples are presented where solutes are retained based on RP, ANP, or dual retention mechanisms. The silica hydride-based stationary phases can also retain compounds in the organic normal phase.