Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview
Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview
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DOI：
10.1002/ar.23843
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发表时间：
2018-10-01
影响因子：
2
通讯作者：
Joukal, Marek
中科院分区：
文献类型：
--
作者：
Dubovy, Petr;Klusakova, Ilona;Joukal, Marek
周围神经损伤导致受影响神经元的深刻改变，这是由内在和外在分子事件之间的相互作用引起的。重新启动神经元再生程序是受损周围神经功能恢复的重要先决条件。背根神经节中的初级感觉神经元及其胞体为研究轴突切断后调节内在神经元再生能力的机制提供了有用的体内和体外模型。这些研究经常需要指出相应神经元的再生状态。我们总结了几个再生相关蛋白的免疫组化检测的关键问题，作为标记的再生程序在大鼠初级感觉神经元和周围神经轴突再生的指标的启动。本综述还包括我们自己的结果GAP 43和SCG 10表达在不同的DRG神经元后，双重免疫染色与神经元亚群的分子标记物（NF 200，CGRP，和IB 4）以及转录因子（ATF 3和激活的STAT 3）后，单侧坐骨神经损伤。Anat Rec，301：1618-1627，2018。(c)2018 Wiley Periodicals，Inc.
Peripheral nerve injury results in profound alterations of the affected neurons resulting from the interplay between intrinsic and extrinsic molecular events. Restarting the neuronal regenerative program is an important prerequisite for functional recovery of the injured peripheral nerve. The primary sensory neurons with their cell bodies in the dorsal root ganglia provide a useful in vivo and in vitro model for studying the mechanisms that regulate intrinsic neuronal regeneration capacity following axotomy. These studies frequently need to indicate the regenerative status of the corresponding neurons. We summarize the critical issues regarding immunohistochemical detection of several regeneration-associated proteins as markers for the initiation of the regeneration program in rat primary sensory neurons and indicators of axon regeneration in the peripheral nerves. This overview also includes our own results of GAP43 and SCG10 expression in different DRG neurons following double immunostaining with molecular markers of neuronal subpopulations (NF200, CGRP, and IB4) as well as transcription factors (ATF3 and activated STAT3) following unilateral sciatic nerve injury. Anat Rec, 301:1618-1627, 2018. (c) 2018 Wiley Periodicals, Inc.