Activation of a Lipase Triggered by Interactions with Supercritical Carbon Dioxide in the Near-Critical Region
Activation of a Lipase Triggered by Interactions with Supercritical Carbon Dioxide in the Near-Critical Region
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近临界区与超临界二氧化碳相互作用引发脂肪酶的激活
DOI：
10.1021/j100022a001
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
H. W. Blanch
中科院分区：
文献类型：
--
作者：
Y. Ikushima;N. Saitǒ;M. Arai;H. W. Blanch
通过傅里叶变换红外光谱结合微重量分析，在压力为 6.86—19.12 MPa、温度为 304.1 K 的条件下，研究了超临界二氧化碳中酶（Candida cylindracea）的二级结构以及二氧化碳与酶分子之间的相互作用。结果发现，在二氧化碳临界点附近非常有限的压力范围内，相互作用大大增加，并引发酶的剧烈构象变化，导致活性位点的出现和催化由油酸和(±)-香茅醇立体选择性合成(5)-(—)-油酸3, 7-二甲基-6-辛烯酯。近临界区域的超临界二氧化碳介质应该通过引起表面基团的移动并创建活性位点来触发酶的激活，从而产生立体选择性机制。
Secondary structures of an enzyme (Candida cylindracea) in supercritical carbon dioxide and interactions operating between carbon dioxide and enzyme molecules were studied by Fourier transform infrared spectroscopy combined with microgravimetry at pressures of 6.86—19.12 MPa and at 304.1 K. It was found that, in very limited pressure range near the critical point of carbon dioxide, the interactions are greatly increased and allowed to provoke drastic conformational changes of the enzyme, causing active sites to emerge and catalyze the stereoselective synthesis of (5)-(—)-oleic acid 3, 7-dimethyl-6-octenyl ester from oleic acid, and (±)-citronellol. Supercritical carbon dioxide medium in the near-critical region should be a trigger to the activation of the enzyme by causing the movement of surface groups and creating an active site, producing a stereoselective machinery.