In situ microscopy for in-line monitoring of the enzymatic hydrolysis of cellulose.
In situ microscopy for in-line monitoring of the enzymatic hydrolysis of cellulose.
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用于在线监测纤维素酶水解的原位显微镜。
DOI：
10.1021/ac4008495
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发表时间：
2013
影响因子：
7.4
通讯作者：
A. Liese
中科院分区：
文献类型：
--
作者：
B. Opitz;A. Prediger;Christian Lüder;Marrit Eckstein;L. Hilterhaus;P. Lindner;S. Beutel;T. Scheper;A. Liese
描述了一种监测纤维素酶水解的新在线方法。使用新的原位显微镜原型，可以无创地测定粒径分布。为了自动分析所获取的图像，开发了一种称为 CelluloseAnalyzer 的新处理算法。它能够跟踪颗粒数量，此外还允许在酶水解反应过程中监测颗粒尺寸部分的比例。使用该技术，观察到内切葡聚糖酶和纤维素酶混合物的水解之间存在显着差异。此外，将原位显微镜结果与激光衍射光谱和凝胶渗透色谱法离线测量的结果进行了比较。
A new in-line method for the monitoring of enzymatic hydrolysis of cellulose is described. Using a new in situ microscope prototype, the noninvasive determination of particle size distributions was possible. For the automated analysis of the acquired images, a new processing algorithm called CelluloseAnalyzer was developed. It enabled tracking of the number of particles and moreover allowed monitoring of the proportions of particle size fractions during the course of enzymatic hydrolysis reactions. Using this technique, significant differences between hydrolysis with endoglucanases and cellulase mixtures were observed. Furthermore, the in situ microscopy results were compared with results from off-line measurements with laser diffraction spectroscopy and gel permeation chromatography.