Hyperglycosylated hCG (Invasive Trophoblast Antigen, ITA) a key antigen for early pregnancy detection
Hyperglycosylated hCG (Invasive Trophoblast Antigen, ITA) a key antigen for early pregnancy detection
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DOI：
10.1016/s0009-9120(03)00108-5
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发表时间：
2003-11-01
影响因子：
2.8
通讯作者：
Stephens, ND
中科院分区：
文献类型：
--
作者：
Cole, LA;Khanlian, SA;Stephens, ND
目的：高糖基化人绒毛膜促性腺激素（hCG）是一种具有超大O-连接寡糖的hCG变体，由绒毛膜癌和妊娠中表型侵袭性细胞滋养层细胞产生。它是妊娠第一周产生的hCG的主要形式。我们调查的重要性，高糖hCG在妊娠试验和检测目前的hCG tests.Design和方法：我们测量了512妊娠整个妊娠期的高糖hCG和总hCG的浓度。我们评估和比较的能力，14个常用的商业实验室BCG测试和18个家庭妊娠测试，以检测定期和高糖基化的hCG。结果：高糖基化的hCG是主要来源的hCG相关的免疫反应性在早期妊娠。在月经缺失后的一周内，高糖基化hCG测量可能比常规hCG测量在检测妊娠方面更敏感。在14种商业实验室hCG检测中，3种适当检测高糖基化hCG标准品。结论：高糖基化hCG可能是检测早孕的关键分子。然而，大多数测试检测不佳或未能检测到这种关键抗原。需要新的妊娠试验，可以单独检测高糖基化hCG，也可以同等检测常规hCG和高糖基化hCG。(C)2003年加拿大临床化学家协会。All rights reserved.
Objectives: Hyperglycosylated human chorionic gonadotrophin (hCG) is an hCG variant with extra-large O-linked oligosaccharides, produced by phenotypically invasive cytotrophoblast cells in choriocarcinoma and pregnancy. It is the principal form of hCG produced in the first weeks of gestation. We investigated the importance of hyperglycosylated hCG in pregnancy testing and its detection by current hCG tests.Design and methods: We measured the concentration of hyperglycosylated hCG and total hCG in 512 pregnancies throughout gestation. We assessed and compared the abilities of 14 commonly used commercial laboratory bCG tests and 18 home pregnancy tests to detect regular and hyperglycosylated hCG.Results: Hyperglycosylated hCG is the principal source of hCG-related immunoreactivity in early pregnancy. In the week following missing menses, hyperglycosylated hCG measurements may be more sensitive than regular hCG measurements in detecting pregnancy. Of 14 commercial laboratory hCG tests, 3 appropriately detected hyperglycosylated hCG standard. Of 18 different home pregnancy products 11 poorly or very poorly detected this key antigen.Conclusions: Hyperglycosylated hCG may be the key molecule in the detection of early pregnancy. However, the majority of tests poorly detected or failed to detect this key antigen. New pregnancy tests are needed that either solely detect hyperglycosylated hCG or equally detect regular hCG and hyperglycosylated hCG. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.