Microfluidic advances in phenotypic antibiotic susceptibility testing.
Microfluidic advances in phenotypic antibiotic susceptibility testing.
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DOI：
10.1007/s10544-016-0121-8
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发表时间：
2016-12
影响因子：
2.8
通讯作者：
Sauer-Budge AF
中科院分区：
文献类型：
--
作者：
Campbell J;McBeth C;Kalashnikov M;Boardman AK;Sharon A;Sauer-Budge AF
抗生素的广泛使用和误用导致了微生物抗生素耐药性的强烈自然选择。尽管造成这场危机的因素有很多，但最重要的因素--广谱抗生素的广泛使用--在很大程度上是由这样一个事实驱动的，即确定抗生素敏感性的标准程序包括1-2天的培养期，结果是从患者样本到最终确定需要48-72小时。显然，解决这个问题需要的是颠覆性的方法，而不是小幅的增量收益。与现有的宏观方法相比，微流控领域具有几个优势，包括：更快的分析、更多的多路传输、更小的体积、更好的便携性、更高的灵敏度和快速的检测方法。这一视角将涵盖过去两年在微流控、表型抗生素敏感性测试(AST)领域取得的进展。根据芯片的功能组织部分-从简单的显微镜平台，到梯度发生器，再到基于抗体的捕获设备。提出了监测生长的微流控AST方法以及那些不基于生长的方法。最后，我们将就该领域仍然面临的主要障碍提出我们的看法，包括需要快速样品制备和负担得起的检测技术。
A strong natural selection for microbial antibiotic resistance has resulted from the extensive use and misuse of antibiotics. Though multiple factors are responsible for this crisis, the most significant factor – widespread prescription of broad-spectrum antibiotics – is largely driven by the fact that the standard process for determining antibiotic susceptibility includes a 1–2-day culture period, resulting in 48–72 hours from patient sample to final determination. Clearly, disruptive approaches, rather than small incremental gains, are needed to address this issue. The field of microfluidics promises several advantages over existing macro-scale methods, including: faster assays, increased multiplexing, smaller volumes, increased portability for potential point-of-care use, higher sensitivity, and rapid detection methods. This Perspective will cover the advances made in the field of microfluidic, phenotypic antibiotic susceptibility testing (AST) over the past two years. Sections are organized based on the functionality of the chip – from simple microscopy platforms, to gradient generators, to antibody-based capture devices. Microfluidic AST methods that monitor growth as well as those that are not based on growth are presented. Finally, we will give our perspective on the major hurdles still facing the field, including the need for rapid sample preparation and affordable detection technologies.