Clostridium thermoaceticum forms methanol from carbon monoxide in the presence of viologen dyes
Clostridium thermoaceticum forms methanol from carbon monoxide in the presence of viologen dyes
复制标题
热乙酸梭菌在紫罗碱染料存在下由一氧化碳形成甲醇
DOI：
10.1111/j.1574-6968.1987.tb02118.x
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发表时间：
1987
影响因子：
2.1
通讯作者：
H. Simon
中科院分区：
文献类型：
--
作者：
H. White;H. Lebertz;I. Thanos;H. Simon
摘要热醋酸梭菌的整个细胞、细胞粗提物和10万×g离心液的上清液在紫精或碳酸钴存在下催化一氧化碳还原为甲醇。如果没有这样的介体，就无法检测到甲醇。反应的最适pH为5时，[5-14C]甲基四氢叶酸只生成14C标记的乙醇，在甲醇中的放射性可以忽略不计。该反应似乎是由一氧化碳脱氢酶催化的。
Abstract Whole cells of Clostridium thermoaceticum , crude extracts of such cells as well as the supernatant of 100 000 × g centrifugations catalyse the reduction of carbon monoxide to methanol in the presence of viologens or cobalt sepulchrate. Without such a mediator methanol could not be detected. The reaction shows a marked optimum at pH 5. The incubation of [5- 14 C]methyltetrahydrofolate led only to the formation of 14 C-labeled ethanol; the radioactivity in methanol was negligible. The reaction seems to be catalysed by carbon monoxide dehydrogenase.