The effect of thermal injury on murine neutrophil oxidative metabolism.
The effect of thermal injury on murine neutrophil oxidative metabolism.
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热损伤对小鼠中性粒细胞氧化代谢的影响。
DOI：
10.1097/00004630-198903000-00005
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hansbrough,JF
中科院分区：
文献类型：
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作者：
Gadd,MA;Hansbrough,JF
多形核白细胞杀死细菌和酵母菌的能力通过细胞化学发光或类似的氧化代谢过程中产生H2 O2来反映。采用流式细胞术和2 '7'二氯二氢荧光素二乙酸酯测定了25%体表面积烧伤后第1、5和10天热损伤对小鼠多形核白细胞氧化代谢的直接影响和烧伤血清对白细胞氧化代谢的间接影响。将对照或烧伤腹膜白细胞和10%对照或烧伤血清与2 ′ 7 ′二氯二氢荧光素-二乙酸酯在体外孵育15分钟，然后用佛波醇12-肉豆蔻酸酯13-乙酸酯刺激。从刺激前后的荧光直方图计算多形核白细胞荧光的变化。烧伤后第5天和第10天，烧伤多形核白细胞的氧化代谢明显受到抑制。在第5天烧伤血清的存在下，控制多形核白细胞产生的H2 O2水平降低，第10天恢复正常。一般来说，热损伤后第5天和第10天的杀菌活性明显降低，可能与脓毒症风险增加有关。
The ability of polymorphonuclear leukocytes to kill bacteria and yeast is reflected by cellular chemiluminescence or similary by the production of H2O2during oxidative metabolism. With the use of flow cytometry and 2'7'dichlorodihydrofluorescein-diacetate, we determined the direct effect of thermal injury and the indirect effect of burn serum on murine polymorphonuclear leukocyte oxidative metabolism after stimulation on days 1, 5, and 10 after 25% total body surface area burn. Control or burn peritoneal leukocytes and 10% control or burn serum were incubated in vitro with 2'7'dichlorodihydrofluorescein-diacetate for 15 minutes, then stimulated with phorbol 12-myristate 13-acetate. The change in polymorphonuclear leukocyte fluorescence was calculated from fluorescence histograms before and after stimulation. The oxidative metabolism of burn polymorphonuclear leukocytes was clearly depressed on days 5 and 10 after burn injury. Control polymorphonuclear leukocytes in the presence of day 5 burn serum produced decreased levels of H2O2, returning to normal by day 10. In general, bactericidal activity is markedly depressed on days 5 and 10 after thermal injury and may be associated with increased risk of sepsis.