The effects L-leucine on the synthesis of urea, glutamate and glutamine by isolated rat liver cells.
The effects L-leucine on the synthesis of urea, glutamate and glutamine by isolated rat liver cells.
复制标题
L-亮氨酸对离体大鼠肝细胞合成尿素、谷氨酸和谷氨酰胺的影响。
作者：
J M Mourão;J. McGivan;J. Chappell
以丙氨酸或15种不同氨基酸的混合物为氮源，加入L-亮氨酸抑制离体大鼠肝细胞尿素的合成。在丙氨酸存在下，亮氨酸促进谷氨酸和谷氨酰胺的产生。亮氨酸对可溶性谷氨酸脱氢酶、线粒体和分离细胞的作用的比较支持亮氨酸通过激活谷氨酸脱氢酶发挥其作用的假设。有人建议，这后一种酶可能不是重要的生产氨甲酰磷酸合成的NH3迄今已被认为。
With either alanine or a mixture of 15 different amino acids as nitrogen source, the addition of L-leucine inhibited the synthesis of urea by isolated rat liver cells. With alanine present leucine promoted the production of glutamate and glutamine. Comparison of effects of leucine on soluble glutamate dehydrogenase, mitochondria and isolated cells supports the postulate that leucine exerts its effect through activation of glutamate dehydrogenase. It is suggested that this latter enzyme may not be as important for the production of NH3 for carbamoyl phosphate synthesis as has been considered hitherto.