HIV-1 Thai B strain has spread out of former plasma donors into general population through sexual contact in Henan, China
HIV-1 Thai B strain has spread out of former plasma donors into general population through sexual contact in Henan, China
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HIV-1 泰国 B 株已通过中国河南省的性接触从前血浆捐献者传播到普通人群
DOI：
10.1002/jmv.24383
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发表时间：
2016-04-01
影响因子：
12.7
通讯作者：
Li, Jingyun
中科院分区：
文献类型：
--
作者：
Li, Lin;Sun, Guoqing;Li, Jingyun
中国河南省的特点是20世纪90年代在前血浆捐献者中爆发了泰国B株艾滋病毒流行。有偿献血被禁止后，泰国B毒株是否会从曾经的血浆捐献者中传播到性传播人群中尚不得而知。为了回答这个问题，研究中使用系统发育分析来探讨在这两个人群中传播的艾滋病病毒毒株的关系。通过性接触感染的 HIV-1 血清阳性未用药患者被纳入该研究。使用巢式 RT-PCR 扩增全长 gag 和 pol 基因，然后进行测序和系统发育分析。还利用可用的pol基因分析了抗HIV药物耐药性的基因型。对 288 名参与者中的 249 名个体进行了 HIV 亚型测定。 B 亚型占主导地位 (202/249, 81.1%)，其次是 CRF01_AE (25/249, 10.0%)、CRF07_BC (14/249, 5.6%)、C (4/249, 1.6%)、URF (3/249, 1.2%) 和 CRF08_BC (1/249, 0.4%）。大多数B亚型菌株属于泰国B谱系。在系统发育树中，在性传播人群中发现的所有泰国 B 毒株与来自前献血者的毒株混合在一起，表明这两个人群中流行的毒株之间存在密切的系统发育关系。 9.9% 的人发现了 TDR。泰国B毒株已从河南省的前献血者中传播出来。这一发现将有助于了解河南省HIV-1的分布和演变，也为行为改变干预提供线索。 J. Med。病毒。 88:614-621，2016 年。(c) 2015 年 Wiley 期刊公司。
Henan, China is characterized by the outbreak of HIV epidemic of Thai B strain in former plasma donors in 1990s. After the forbidden of paid blood donation, whether Thai B strain will spread out of former plasma donors into sexual transmitted population is unknown. To answer the question, phylogenetic analysis was used to explore relationships of HIV strains circulating in those two populations in the study. HIV-1 sero-positive drug-naive patients infected through sexual contact were enrolled into the study. Full length gag and pol genes were amplified with nested RT-PCR followed by sequencing and phylogenetic analysis. The genotypes of anti-HIV drug resistance were also analyzed with available pol genes. HIV subtypes were determined in 249 individuals from 288 participants. Subtype B was dominant (202/249, 81.1%), followed by CRF01_AE (25/249, 10.0%), CRF07_BC (14/249, 5.6%), C (4/249, 1.6%), URF (3/249, 1.2%), and CRF08_BC (1/249, 0.4%). Most of subtype B strains belong to Thailand B lineage. All of Thai B strains identified in sexual transmitted population intermixed with those from former blood donors in phylogenetic tree, suggesting close phylogenetic relationship between strains epidemic in those two populations. TDR was identified in 9.9% individuals. Thai B strain has spread out of former blood donors in Henan province. The finding will contribute to understanding the distribution and evolution of HIV-1 in Henan province and also provide clue to behavior change intervention. J. Med. Virol. 88:614-621, 2016. (c) 2015 Wiley Periodicals, Inc.