Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development
Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development
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DOI：
10.1006/dbio.1999.9586
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发表时间：
2000-02-15
影响因子：
2.7
通讯作者：
Francis-West, PH
中科院分区：
文献类型：
--
作者：
Ladher, RK;Church, VL;Francis-West, PH
已知Wnt基因在许多胚胎结构的形成和发育过程中发挥重要作用。Wnt的受体是卷曲蛋白家族的成员，其含有结合Wnt蛋白的富含半胱氨酸的结构域（CRD）。最近，已经鉴定了几种也含有CRD的分泌型卷曲相关蛋白（Sfrps），其中一些可以结合并拮抗Wnt蛋白。在本文中，我们报告的表达模式的鸡同源物Frzb，一个已知的Wnt拮抗剂，和Sfrp-2。这两种基因都在已知Wnt在发育中发挥作用的区域表达，包括神经管、肌节、软骨和上皮-间充质相互作用的位点。最初，Sfrp-2和Frzb在神经板和神经管中的重叠区域中表达，而后来，它们具有不同的模式。特别是Sfrp-2与肌肉发生有关，而Frzb与软骨发生有关，表明它们在发育过程中发挥不同的作用。最后，我们使用早期非洲爪蟾胚胎作为体内试验，以表明Sfrp-2，像Frzb一样，是Wnt拮抗剂。这些结果表明，Sfrp-2和Frzb可能通过调节Wnt信号在发育中的胚胎中发挥作用。(C)北京大学出版社.
The Wnt genes are known to play fundamental roles during patterning and development of a number of embryonic structures. Receptors for Wnts are members of the Frizzled family of proteins containing a cysteine-rich domain (CRD) that binds the Wnt protein. Recently several secreted frizzled-related proteins (Sfrps) that also contain a CRD have been identified and some of these can both bind and antagonise Wnt proteins. In this paper we report the expression patterns of the chick homologues of Frzb, a known Wnt antagonist, and Sfrp-2. Both genes are expressed in areas where Wnts are known to play a role in development, including the neural tube, myotome, cartilage, and sites of epithelial-mesenchymal interactions. Initially, Sfrp-2 and Frzb are expressed in overlapping areas in the neural plate and neural tube, whereas later, they have distinct patterns. In particular Sfrp-2 is associated with myogenesis while Frzb is associated with chondrogenesis, suggesting that they play different roles during development. Finally, we have used the early Xenopus embryo as an in vivo assay to show that Sfrp-2, like Frzb, is a Wnt antagonist. These results suggest that Sfrp-2 and Frzb may function in the developing embryo by modulating Wnt signalling. (C) 2000 Academic Press.