A compact semi-automated continuous cultivation system for Plasmodium falciparum.
A compact semi-automated continuous cultivation system for Plasmodium falciparum.
复制标题
一种紧凑型半自动恶性疟原虫连续培养系统。
DOI：
10.1016/0035-9203(81)90338-2
复制
发表时间：
1981
影响因子：
2.2
通讯作者：
J. Williamson
中科院分区：
文献类型：
--
作者：
T. Scott;R. Wilson;J. Williamson
描述了一种紧凑、廉价的恶性疟原虫半自动连续培养系统。该系统操作简单，可以在无人值守的情况下进行几天的培养。一个特别有用的特征是便携式培养模块，其可自由地从培养箱转移到组织培养柜，用于手动操作，例如红细胞的取样或添加。每个培养模块都装有塑料瓶，可提供高达500平方厘米的总培养表面积。如果每天更换培养基，在红细胞密度为1 - 7 × 108/ml时，通常可获得12%的寄生虫血症。
A compact inexpensive semi-automated continuous cultivation system forPlasmodium falciparumis described. The system is simple to operate and allows cultures to proceed unattended for several days. A particularly useful feature is a portable cultivation module that is freely transferable from the incubator to a tissue culture cabinet for manual operations such as sampling or addition of red cells. Each cultivation module holds plastic flasks which can give a combined culture surface area of up to 500 cm2. Parasitaemias of 12% are attained routinely at red cell densities of 1 to 7 × 108per ml, if the medium is changed daily.