Preliminary biochemical characterization of the novel, non-AT1, non-AT2 angiotensin binding site from the rat brain.
Preliminary biochemical characterization of the novel, non-AT1, non-AT2 angiotensin binding site from the rat brain.
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DOI：
10.1007/s12020-010-9328-2
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发表时间：
2010-06
期刊：
影响因子：
3.7
通讯作者：
Speth, Robert C.
中科院分区：
文献类型：
--
作者：
Karamyan, Vardan T.;Arsenault, Jason;Escher, Emanuel;Speth, Robert C.
关键词：
最近在存在巯基反应性血管紧张素酶抑制剂对氯汞苯甲酸盐的情况下在脑膜中发现了血管紧张素II和III的新结合位点。该结合位点明显不同于其他已知的血管紧张素受体：AT 1、AT 2、AT 4和mas癌基因蛋白（Ang 1-7受体）。初步的生化表征研究已经完成了对这种蛋白质的交联与125碘标记的光亲和探针和溶解的放射性标记的结合位点。聚丙烯酰胺凝胶电泳研究和等电聚焦表明，该膜结合位点是一种分子量为70-85 kDa，等电点为~7的蛋白质。溴化氰水解的蛋白质产生两个放射性标记的片段12.5和25 kDa。基于PNGaseF未能改变其在7.5%聚丙烯酰胺凝胶上的迁移速率，该蛋白似乎未被N-糖基化。血管紧张素II与该蛋白的结合不受GTPγS或Gpp（NH）p的影响，表明它不是G蛋白偶联受体。进一步的表征研究旨在鉴定该蛋白质作为新型血管紧张素受体、血管紧张素清除剂（清除受体）或血管紧张素酶。
A novel binding site for angiotensins II and III was recently discovered in brain membranes in the presence of the sulfhydryl reactive angiotensinase inhibitor parachloromercuribenzoate. This binding site is distinctly different from the other known receptors for angiotensins: AT1, AT2, AT4, and mas oncogene protein (Ang 1-7 receptor). Preliminary biochemical characterization studies have been done on this protein by crosslinking it with 125I-labeled photoaffinity probes and solubilizing the radiolabeled binding site. Polyacrylamide gel electrophoresis studies and isoelectric focusing indicate that this membrane bound binding site is a protein with a molecular weight of 70–85 kDa and an isoelectric point of ~7. Cyanogen bromide hydrolysis of the protein yielded two radiolabeled fragments of 12.5 and 25 kDa. The protein does not appear to be N-glycosylated based upon the failure of PNGaseF to alter its migration rate on a 7.5% polyacrylamide gel. The binding of angiotensin II to this protein is not affected by GTPγS or Gpp(NH)p, suggesting that it is not a G protein-coupled receptor. Further characterization studies are directed to identify this protein either as a novel angiotensin receptor, an angiotensin scavenger (clearance receptor) or an angiotensinase.
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影响因子：
4.8
作者：
Clément, M;Martin, SS;Escher, E
通讯作者：
Escher, E
影响因子：
2.9
作者：
Karamyan, Vardan T.;Speth, Robert C.
通讯作者：
Speth, Robert C.
影响因子：
2.1
作者：
Arsenault, J.;Renaud, M. P. La Haye;Escher, E.
通讯作者：
Escher, E.
影响因子：
--
作者：
BOSSE, R;SERVANT, G;ESCHER, E
通讯作者：
ESCHER, E
影响因子：
--
作者：
CARSON, MC;HARPER, CML;CATT, KJ
通讯作者：
CATT, KJ