Gut Microbiota Are Associated With Psychological Stress-Induced Defections in Intestinal and Blood-Brain Barriers
Gut Microbiota Are Associated With Psychological Stress-Induced Defections in Intestinal and Blood-Brain Barriers
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DOI：
10.3389/fmicb.2019.03067
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发表时间：
2020-01-15
影响因子：
5.2
通讯作者：
Zhang, Jingtao
中科院分区：
文献类型：
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作者：
Geng, Shaohui;Yang, Liping;Zhang, Jingtao
在心理压力期间已经发现了改变的肠道微生物群，这导致了全球严重的健康问题。肠屏障和血脑屏障的完整性调节细菌移位的过程，并可以为神经系统提供有关环境的实时信息。然而，肠道微生物群与心理压力的关系仍有待充分解释。本研究采用改良的通讯盒建立心理应激模型，比较模型组和对照组大鼠小肠（十二指肠、空肠、回肠）和血脑屏障（杏仁核、海马）多个区域紧密连接蛋白的表达。我们还使用16 S rRNA基因测序进行了粪便微生物群分析。与对照组相比，模型组大鼠应激相关指标促肾上腺皮质激素、NR 3C 1，2和去甲肾上腺素的表达水平升高。心理压力降低了大脑和肠道紧密连接蛋白的水平，包括claudin 5，occludin，α-肌动蛋白和ZO-1。微生物区系分析显示，模型组的微生物多样性和肠单胞菌、链状球菌和球尾菌的粪便比例升高。进一步的分析表明Allisonella和Odoribacter的负相关性，以及norank_f__Peptococcaceae、Clostridium_sensu_stricto_1和Coprococcus_2与claudin 5、occludin、α-肌动蛋白和ZO-1的正相关性。我们使用啮齿动物模型来探索在心理应激下肠道和血脑屏障受损与粪便微生物群改变之间的关系，这提高了我们对肠脑轴的理解。在这里，线索汇聚在一起，控制肠道和大脑的基本发育过程，如屏障功能。本研究为探讨情绪障碍的发病机制和制定临床治疗方案提供了新的方向。
Altered gut microbiota has been identified during psychological stress, which causes severe health issues worldwide. The integrity of the intestinal barrier and blood-brain barrier regulates the process of bacterial translocation and can supply the nervous system with real-time information about the environment. However, the association of gut microbiota with psychological stress remains to be fully interpreted. In this study, we established a psychological stress model using an improved communication box and compared the expression of tight junction proteins in multiple regions of the intestinal (duodenum, jejunum, ileum) and blood-brain (amygdala, hippocampus) barriers between model and control rats. We also conducted fecal microbiota analysis using 16S rRNA gene sequencing. Expression levels of the stress-related indicators adrenocorticotropic hormone, NR3C1,2, and norepinephrine were increased in the model group compared to control group. Psychological stress reduced brain and intestinal levels of tight junction proteins, including claudin5, occludin, alpha-actin, and ZO-1. Microbiota analysis revealed elevated microbial diversity and fecal proportions of Intestinimonas, Catenisphaera, and Globicatella in the model group. Further analysis indicated a negative correlation of Allisonella and Odoribacter, as well as a positive correlation of norank_f__Peptococcaceae, Clostridium_sensu_stricto_1, and Coprococcus_2, with claudin5, occludin, alpha-actin, and ZO-1. Our use of a rodent model to explore the association between compromised intestinal and blood-brain barriers and altered fecal microbiota under psychological stress improves our understanding of the gut-brain axis. Here, cues converge to control basic developmental processes in the intestine and brain such as barrier function. This study provides new directions for investigating the pathogenesis of emotional disorders and the formulation of clinical treatment.