Diversity of Babesia infecting European sheep ticks (Ixodes ricinus)
Diversity of Babesia infecting European sheep ticks (Ixodes ricinus)
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DOI：
10.1128/jcm.39.9.3395-3397.2001
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发表时间：
2001-09-01
影响因子：
9.4
通讯作者：
Avsic-Zupanc, T
中科院分区：
文献类型：
--
作者：
Duh, D;Petrovec, M;Avsic-Zupanc, T
采用基于核小亚基rRNA基因（nss-核糖体DNA [rDNA]）的PCR检测方法，对在斯洛文尼亚各地采集的蓖麻伊蚊（蜱螨：伊蚊科）成虫和雌雄蜱进行了巴贝虫寄生虫检测。135只蜱中有13只被发现含有巴贝虫DNA。对nss-rDNA扩增片段的序列测定和分析表明，nss-rDNA与微小巴贝斯虫同源，与臭虫巴贝斯虫和巴贝斯虫发散型巴贝斯虫高度同源。本研究结果首次提供了欧洲蓖麻蜱中存在微布氏菌和异布氏菌样寄生虫的遗传证据。
Questing Ixodes ricinus (Acari: Ixodidae) adult and nymphal ticks collected in various parts of Slovenia were tested for the presence of babesial parasites with a PCR assay based on the nuclear small subunit rRNA gene (nss-ribosomal DNA [rDNA]). Thirteen of 135 ticks were found to contain babesial DNA. Sequence determination and analysis of amplified portions of nss-rDNA revealed their identity with Babesia microti and a high degree of homology with Babesia odocoilei and Babesia divergens. The results of this study represent the first genetic evidence of B. microti and B. divergens-like parasites in I. ricinus ticks in Europe.