Fungal endophytes promote the accumulation of Amaryllidaceae alkaloids in. . Lycoris radiata
Fungal endophytes promote the accumulation of Amaryllidaceae alkaloids in. . Lycoris radiata
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真菌内生菌促进石蒜科生物碱的积累。
DOI：
10.1111/1462-2920.14958
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发表时间：
2020
影响因子：
5.1
通讯作者：
Ren Wang
中科院分区：
文献类型：
--
作者：
Jiayu Zhou;Zhilin Liu;Songfeng Wang;Jie Li;Yikui Li;Wei‐Kang Chen;Ren Wang
石蒜是石蒜科生物碱的主要来源，但石蒜生物碱在植物体内的含量较低，限制了其药用开发利用。内生真菌能促进植物次生代谢产物的积累。在这项研究中，我们分析了可培养的真菌内生菌在不同器官的L.辐射的多样性。然后，通过分析每个真菌物种的检出率和每个测试的生物碱的含量之间的相关性，我们提出了几个真菌候选人牵连的生物碱积累的增加。这一点通过将这些候选者转移到L. radiataplants中得到了验证。根据2018年5月和2019年10月进行的两项独立实验的结果，单独接种9种真菌内生菌显著增加了整个辐射L.植物中受试生物碱的总含量。这是首次在辐射乳杆菌中发现内生真菌能够促进多种生物碱的积累。因此，我们的研究结果提供了更好地了解植物和真菌内生菌之间的相互作用的见解，并提出了一个有效的策略，提高生物碱含量的培养ofL.radiata。
Lycoris radiatais a main source of Amaryllidaceae alkaloids; however, the low content of these alkaloidsin plantaremains a limit to their pharmaceutical development and utilization. The accumulation of secondary metabolites can be enhanced in plants inoculated with fungal endophytes. In this study, we analysed the diversity of culturable fungal endophytes in different organs ofL.radiata. Then, by analysing the correlation between the detectable rate of each fungal species and the content of each tested alkaloid, we proposed several fungal candidates implicated in the increase of alkaloid accumulation. This was verified by inoculating these candidates toL.radiataplants. Based on the results of two independent experiments conducted in May 2018 and October 2019, the individual inoculation of nine fungal endophytes significantly increased the total content of the tested alkaloids in the entireL.radiataplants. This is the first study inL.radiatato show that fungal endophytes are able to improve the accumulation of various alkaloids. Therefore, our results provide insights into a better understanding of interactions between plants and fungal endophytes and suggest an effective strategy for enhancing the alkaloid content in the cultivation ofL.radiata.