Cellular Defense and Sensory Cell Survival Require Distinct Functions of ebi in Drosophila.
Cellular Defense and Sensory Cell Survival Require Distinct Functions of ebi in Drosophila.
复制标题
DOI：
10.1371/journal.pone.0141457
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tsuda L
中科院分区：
文献类型：
--
作者：
Lim YM;Yagi Y;Tsuda L
先天免疫反应和应激诱导的细胞凋亡是与细胞防御相关的成熟信号通路。 NF-κB 和 AP-1 是氧化还原敏感转录因子，在这些途径中发挥重要作用。在这里，我们发现 Ebi 是哺乳动物共阻遏分子转导蛋白 β-like 1 (TBL1) 的果蝇同源物，它以多种方式调节氧化还原敏感转录因子靶标的特定基因的表达。为了响应不同的刺激，Ebi 激活基因表达以支持脂肪体中的急性免疫反应，而 Ebi 则抑制参与感光细胞凋亡的基因。因此，Ebi 似乎充当基因的调节开关，这些基因响应不同的外部刺激而被激活或抑制。我们的结果提供了明确的体内证据，表明含有 Ebi 的共阻遏物复合物以独特的方式发挥作用来调节转录，而转录是调节果蝇发育过程中各种过程的输出所必需的。
The innate immune response and stress-induced apoptosis are well-established signaling pathways related to cellular defense. NF-κB and AP-1 are redox-sensitive transcription factors that play important roles in those pathways. Here we show that Ebi, a Drosophila homolog of the mammalian co-repressor molecule transducin β-like 1 (TBL1), variously regulates the expression of specific genes that are targets of redox-sensitive transcription factors. In response to different stimuli, Ebi activated gene expression to support the acute immune response in fat bodies, whereas Ebi repressed genes that are involved in apoptosis in photoreceptor cells. Thus, Ebi seems to act as a regulatory switch for genes that are activated or repressed in response to different external stimuli. Our results offer clear in vivo evidence that the Ebi-containing co-repressor complex acts in a distinct manner to regulate transcription that is required for modulating the output of various processes during Drosophila development.