FRET templated by G-quadruplex DNA: A specific ternary interaction using an original pair of donor/acceptor partners
FRET templated by G-quadruplex DNA: A specific ternary interaction using an original pair of donor/acceptor partners
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DOI：
10.1021/ja062193h
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发表时间：
2006-09-13
影响因子：
15
通讯作者：
Teulade-Fichou, Marie-Paule
中科院分区：
文献类型：
--
作者：
Allain, Clemence;Monchaud, David;Teulade-Fichou, Marie-Paule
G-quadruplex represents a suitable scaffold for FRET (fluorescence resonance energy transfer) since its two external quartets offer two well-defined binding sites for concomitant trapping of donor/acceptor partners. Combining selective G-quadruplex binders (macrocyclic bis(quinacridine) BOQ(1) or monomeric quinacridine MMQ(1), donor) with a highly fluorescent DNA probe (thiazole orange, acceptor), we designed a structure-specific FRET-system based on an unprecedented noncovalent ternary complex. This system could be potentially usable as a signature for quadruplex-DNA conformation in solution, but also might offer a unique means for observing cation and ligand binding influence on quadruplex topology.
