Analysis of thymic stromal cell populations using flow cytometry
Analysis of thymic stromal cell populations using flow cytometry
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DOI：
10.1016/s0022-1759(01)00493-8
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发表时间：
2002-02-01
影响因子：
2.2
通讯作者：
Boyd, RL
中科院分区：
文献类型：
--
作者：
Gray, DHD;Chidgey, AP;Boyd, RL
淋巴细胞和胸腺基质细胞的异质性反映了淋巴基质相互作用的复杂性，而淋巴基质相互作用对字母表细胞的发育至关重要。虽然单克隆抗体已经描述了这些微环境的许多细胞成分，但使用流式细胞术量化基质细胞亚群的方法仍然定义不清。本研究改进和比较了各种基质细胞分离方法，并确定了各种消化酶对重要表面分子的影响。三色和四色流式细胞术用于关联已建立的和新的基质细胞标记，以定义胸腺成纤维细胞、上皮和表达MHC II类的胸腺内皮的独特亚群。本工作为进一步纯化胸腺基质细胞进行表型、功能和遗传分析奠定了基础。(C) 2002 Elsevier Science B.V.版权所有
The complexity of the lymphostromal interplay that is essential to alphabetaT-cell development is reflected by the heterogeneity of both lymphocytes and thymic stromal cells. While panels of monoclonal antibodies have described many of the cellular components of these microenvironments, the means to quantify stromal cell subsets using flow cytometry remains poorly defined. This study refines and compares various stromal cell isolation procedures and determines the effects of various digestion enzymes on important surface molecules. Three- and four-color flow cytometry is used to correlate established and novel stromal cell markers to define thymic fibroblasts, epithelium and a unique subset of thymic endothelium that express MHC class II. This work provides a basis for the purification of thymic stromal cells for further phenotypic, functional and genetic analysis. (C) 2002 Elsevier Science B.V. All rights reserved.