Thermal annealed silk fibroin membranes for periodontal guided tissue regeneration
Thermal annealed silk fibroin membranes for periodontal guided tissue regeneration
复制标题
DOI：
10.1007/s10856-019-6225-y
复制
发表时间：
2019-02-01
影响因子：
3.7
通讯作者：
Oliveira, Ana L.
中科院分区：
文献类型：
--
作者：
Geao, Catarina;Costa-Pinto, Ana R.;Oliveira, Ana L.
引导组织再生（GTR）是一种应用于牙周缺损重建的外科手术，其中使用闭塞膜来防止快速生长的结缔组织迁移到缺损处。在这项工作中，开发了丝素蛋白（SF）膜用于牙周引导组织再生。以高达 30% 的多种重量比制备 SF 与甘油 (GLY) 或聚乙烯醇 (PVA) 的溶液，然后进行溶剂浇铸并在 85 摄氏度下热退火 6 小时和 12 小时，以生产高柔性和稳定的膜。对它们的形态、物理完整性、化学结构、机械和热性能、溶胀能力和体外降解行为进行了表征。开发的混合膜表现出高延展性，考虑到物理处理的需要和对缺陷的适应性，这一点尤其重要。此外，膜与人牙周膜成纤维细胞 (hPDL) 一起培养长达 7 天。此外，这些共混物的更高亲水性和随之而来的体外蛋白水解降解性优于纯丝素蛋白膜。特别是 SF/GLY 混合物被证明能够支持高细胞粘附和活力，并具有足够的 hPDL 形态，使其成为牙周再生应用的绝佳候选者。[图表]。
Guided tissue regeneration (GTR) is a surgical procedure applied in the reconstruction of periodontal defects, where an occlusive membrane is used to prevent the fast-growing connective tissue from migrating into the defect. In this work, silk fibroin (SF) membranes were developed for periodontal guided tissue regeneration. Solutions of SF with glycerol (GLY) or polyvinyl alcohol (PVA) where prepared at several weight ratios up to 30%, followed by solvent casting and thermal annealing at 85 degrees C for periods of 6 and 12h to produce high flexible and stable membranes. These were characterized in terms of their morphology, physical integrity, chemical structure, mechanical and thermal properties, swelling capability and in vitro degradation behavior. The developed blended membranes exhibited high ductility, which is particular relevant considering the need for physical handling and adaptability to the defect. Moreover, the membranes were cultured with human periodontal ligament fibroblast cells (hPDLs) up to 7 days. Also, the higher hydrophilicity and consequent in vitro proteolytic degradability of these blends was superior to pure silk fibroin membranes. In particular SF/GLY blends demonstrated to support high cell adhesion and viability with an adequate hPDLs' morphology, make them excellent candidates for applications in periodontal regeneration.[GRAPHICS].