LYMPHOCYTE SUBPOPULATIONS IN THE BLADDER WALL IN NORMAL BLADDER, BACTERIAL CYSTITIS AND INTERSTITIAL CYSTITIS
LYMPHOCYTE SUBPOPULATIONS IN THE BLADDER WALL IN NORMAL BLADDER, BACTERIAL CYSTITIS AND INTERSTITIAL CYSTITIS
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DOI：
10.1111/j.1464-410x.1994.tb07635.x
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发表时间：
1994-05-01
期刊：
影响因子：
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通讯作者：
CHRISTMAS, TJ
中科院分区：
文献类型：
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作者：
CHRISTMAS, TJ
目的探讨正常人、细菌性膀胱炎（BC）和间质性膀胱炎（IC）患者膀胱壁淋巴细胞亚群的分布。使用针对T细胞标志物CD 3、CD 4、CD 8、TCR δ 1、δ TCS 1、β F1和IML的单克隆抗体对活检组织进行免疫荧光染色。使用伊加、IgG和IgM的单克隆抗体鉴定浆细胞。确定细胞的位置和数量。结果间质性膀胱炎患者膀胱活检组织中T细胞（CD 4+和CD 8+）的数量明显高于正常对照组，主要分布于膀胱黏膜和尿道黏膜下层，而逼尿肌中无T细胞。间质性膀胱炎患者和细菌性膀胱炎患者的活检组织中T细胞数量没有差异。然而，γ δ T细胞的存在与间质性膀胱炎显著相关。伊加+和IgM+浆细胞位于膀胱尿道和粘膜下层，但不逼尿肌在IC患者和本在这些患者的数量显着大于与BC或正常对照组。结论IC患者尿道及粘膜下CD 4+、CD 8+、γ δ T细胞及伊加+、IgG+、IgM+浆细胞数量增加，提示这些细胞在IC的发病中起积极作用。先前描述的异常尿路上皮表达的HLA-DR和I类分子的高表达与IC患者可能使这些细胞导致破坏尿路上皮-终末期TC。
Objective To identify lymphocyte sub-populations within the bladder wall in normal controls, in patients with bacterial cystitis (BC) or interstitial cystitis (IC).Patients and methods Bladder biopsies were taken from 21 patients with IC, four with BC and five normal controls. Immunofluorescent staining of biopsies was performed using monoclonal antibodies to the T cell markers CD3, CD4, CD8, TCR delta 1, delta TCS1, beta F1 and IML. Plasma cells were identified using monoclonal antibodies to IgA, IgG and IgM. The location and numbers of cells were determined. Peripheral blood lymphocyte counts were also performed in each case.Results T cells (CD4+ and CD8+) were significantly more abundant within the bladder biopsies from patients with interstitial cystitis than in those from the normal controls, and were present within the urothelium and submucosa but not the detrusor. There was no difference between the numbers of T cells present in the biopsies from patients with interstitial cystitis and in those with bacterial cystitis. However, the presence of gamma delta T cells was significantly associated with interstitial cystitis. IgA+ and IgM+ plasma cells were located within the urothelium and submucosa but not the detrusor in patients with IC and the numbers present in these patients were significantly greater than in those with BC or the normal controls. The peripheral blood lymphocyte counts were not altered in any of the study cases.Conclusion The increased numbers of CD4+, CD8+ and gamma delta T cells as well as IgA+, IgG+ and IgM+ plasma cells within the urothelium and submucosa in patients with IC suggest that these cells play an active role in the pathogenesis of the disorder. The previously described abnormal urothelial expression of HLA-DR and hyperexpression of Class I molecules in patients with IC may enable these cells to cause destruction of the urothelium - the end stage of TC.