Plasma low-molecular weight fluorescence in type 1 diabetes mellitus
Plasma low-molecular weight fluorescence in type 1 diabetes mellitus
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DOI：
10.1196/annals.1333.074
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发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Jenkins, AJ
中科院分区：
文献类型：
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作者：
Januszewski, AS;Thomas, MC;Jenkins, AJ
在实验性糖尿病模型中，特征性组织荧光与晚期糖基化终产物（AGE）积累相关，但其在I型糖尿病患者中的效用仍有待确定。我们研究了148名1型糖尿病患者和77名年龄匹配的健康对照者。在禁食过夜后获得的血浆样品中估计低分子量（LMW）荧光团水平。批内和批间变异系数分别为4.7%和6.4%。糖尿病患者的LMW荧光团水平显著高于对照受试者（6.3 +/- 0.6 vs. 4.1 +/- 0.3; P = 0.007）。然而，所有这些差异均来自微血管并发症患者（n = 67，7.5 +/- 1.3）。无并发症患者（4.4 ± 0.2）和对照受试者之间的LMW荧光无差异（P > 0.05）。在多变量分析中，LMW荧光团与肾功能指标相关（P < 0.05），但与糖尿病本身无关。此外，LMW荧光团与氧化应激或全身炎症标志物之间没有相关性。需要进行纵向和干预性研究，以确定LMW荧光团与肾病之间的关联是因果关系。
Characteristic tissue fluorescence is associated with advanced glycation end product (AGE) accumulation in experimental diabetes models, but its utility in patients with type I diabetes remains to be established. We studied 148 patients with type 1 diabetes and 77 healthy age-matched control subjects. Low-molecular weight (LMW) fluorophore levels were estimated in plasma samples obtained after an overnight fast. Intra- and interassay coefficients of variation were 4.7% and 6.4%, respectively. LMW fluorophore levels were significantly higher in patients with diabetes than in control subjects (6.3 +/- 0.6 vs. 4.1 +/- 0.3; P = 0.007). However, all of this difference came from patients with microvascular complications (n = 67, 7.5 +/- 1.3). There was no difference in LMW fluorescence between complication-free patients (4.4 +/- 0.2) and control subjects (P > 0.05). On multivariate analysis, LMW fluorophores correlated with measures of renal function (P < 0.05) but not with diabetes per se. In addition, there was no correlation between LMW fluorophores and the markers of oxidative stress or systemic inflammation. Longitudinal and interventional studies are required to determine whether the association between LMW fluorophores and nephropathy is cause or effect.