Functional role of laminin carbohydrate
Functional role of laminin carbohydrate
复制标题
层粘连蛋白碳水化合物的功能作用
DOI：
--
复制
发表时间：
1984
影响因子：
5.3
通讯作者：
C. Howe
中科院分区：
文献类型：
--
作者：
C. Howe
先前的研究表明，tunicamycin抑制层粘连蛋白的糖基化。本研究利用tunicamycin抑制糖基化作用，研究了糖基化在层粘连蛋白分泌和层粘连蛋白亚基二硫键中的作用。Tunicamycin广泛抑制层粘连蛋白分泌到培养基和细胞外基质中，即使处理细胞中层粘连蛋白的浓度高于对照细胞。在tunicamycin存在下合成的层粘连蛋白亚基为二硫键。因此，抑制糖基化不会对亚基的二硫键产生不利影响，但会减少层粘连蛋白的分泌。糖苷酶也被用来去除层粘连蛋白中的碳水化合物，以研究碳水化合物在层粘连蛋白稳定性及其与另一种细胞外基质成分肝素的相互作用中的作用。糖苷酶脱除约73%的[3H]氨基葡萄糖。糖苷酶处理和未处理的层粘连蛋白在细胞裂解液或培养基中孵育时都是稳定的。糖苷酶处理的层粘连蛋白与未处理的层粘连蛋白一样有效地结合肝素。这些结果表明，碳水化合物部分的存在，至少在未经处理的层粘连蛋白中发现的水平，对于与肝素结合或保护层粘连蛋白免受细胞或培养基中的蛋白水解降解并不是必需的。
Previous work showed that tunicamycin suppresses glycosylation of laminin. In the present work, the role of glycosylation in the secretion of laminin and in the disulfide bonding of laminin subunits was studied, using tunicamycin to inhibit glycosylation. Tunicamycin inhibited extensively the secretion of laminin into culture medium and extracellular matrix even though the treated cells contained higher concentrations of laminin than the control cells. The laminin subunits synthesized in the presence of tunicamycin were disulfide bonded. Thus, suppression of glycosylation did not adversely affect disulfide bonding of the subunits, but did decrease the secretion of laminin. Glycosidases were also used to remove the carbohydrate of laminin to study the role of carbohydrate in the stability of laminin and in its interaction with another extracellular matrix component, heparin. The glycosidases removed about 73% of [3H]glucosamine. Both glycosidase-treated and untreated laminin were stable when incubated with cell lysate or culture medium. The glycosidase-treated laminin bound as efficiently as the untreated laminin to heparin. These results suggest that the presence of a carbohydrate moiety, at least at the level found in untreated laminin, is not essential in binding to heparin or in protecting laminin from proteolytic degradation in the cell or culture medium.
来自畸胎癌来源的壁层内胚层细胞的层粘连蛋白的三个亚基的结构分析。
影响因子：
2.7
作者：
Howe,CC;Dietzschold,B
通讯作者：
Dietzschold,B