Molecular phylogenetic analysis of a taxonomically unstable ranid from Sumatra, Indonesia, reveals a new genus with gastromyzophorous tadpoles and two new species
Molecular phylogenetic analysis of a taxonomically unstable ranid from Sumatra, Indonesia, reveals a new genus with gastromyzophorous tadpoles and two new species
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DOI：
10.3897/zse.94.22120
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发表时间：
2018-03-09
影响因子：
2
通讯作者：
Haas, Alexander
中科院分区：
文献类型：
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作者：
Arifin, Umilaela;Smart, Utpal;Haas, Alexander
腹部吸盘（腹吸盘）的存在使得某些无尾类的蝌蚪能够生活在快速流动的溪流中。腹肢蝌蚪在无尾类中很少见，只在某些美洲蟾蜍和亚洲蛙类中发现。到目前为止，居住在瀑布中的Huia sumatrana是已知的唯一拥有胃Myzophorous蝌蚪的Summerranid。在没有对成虫和幼虫进行彻底取样和分子条形码编码的情况下，还有待确认是否有生活在类似栖息地的其他苏门答腊物种，即，Chalcorana crassiovis，具有这种幼虫类型。此外，该物种的分类地位长期以来一直不确定，其在蛙科的分类地位，以前完全基于形态特征，一直没有得到解决。为了研究这些蛙类的多样性和亲缘关系，并确定苏门答腊新采集的胃潜蝌蚪的身份，我们比较了苏门答腊蛙的基因序列。在苏门答腊岛的许多地方发现了类似crassiovis的分类群。我们对线粒体（12S rRNA，16S rRNA和tRNAval）和核（RAG1和TYR）基因片段的串联数据集进行了系统发育分析和最大似然系统发育分析。我们的分析恢复了C。crassiovis与Clinotarsus，Huia和Meristogenys有关。胃迷走蝌蚪的DNA条形码与来自相同地点的成年人相匹配。在此，我们提供了一个重新描述的成人C。crassiovis提出了“C. kampeni”作为该物种的同义词。分子证据，形态特征和分布表明存在两个相关的新种。这两个新种和C. crassiovis共同代表了一个独特的系统发育分支，具有独特的分子和形态学的synapomorphies，从而重新划分一个新属。
The presence of an adhesive abdominal sucker (gastromyzophory) allows tadpoles of certain species of anurans to live in fast-flowing streams. Gastromyzophorous tadpoles are rare among anurans, known only in certain American bufonids and Asian ranids. To date, Huia sumatrana, which inhabits cascading streams, has been the only Sumatran ranid known to possess gastromyzophorous tadpoles. In the absence of thorough sampling and molecular barcoding of adults and larvae, it has remained to be confirmed whether other Sumatran ranid species living in similar habitats, i.e., Chalcorana crassiovis, possesses this larval type. Moreover, the taxonomic status of this species has long been uncertain and its taxonomic position within the Ranidae, previously based exclusively on morphological characters, has remained unresolved. To study the diversity and relationships of these frogs and to establish the identity of newly collected gastromyzophorous tadpoles from Sumatra, we compared genetic sequences of C. crassiovis-like taxa from a wide range of sites on Sumatra. We conducted bayesian and maximum likelihood phylogenetic analyses on a concatenated dataset of mitochondrial (12S rRNA, 16S rRNA, and tRNAval) and nuclear (RAG1 and TYR) gene fragments. Our analyses recovered C. crassiovis to be related to Clinotarsus, Huia, and Meristogenys. The DNA barcodes of the gastromyzophorous tadpoles matched adults from the same sites. Herein, we provide a re-description of adult C. crassiovis and propose "C. kampeni" as a synonym of this species. The molecular evidence, morphological features, and distribution suggest the presence of two related new species. The two new species and C. crassiovis together represent a distinct phylogenetic clade possessing unique molecular and morphological synapomorphies, thus warranting a new genus.