Development of r4 Gateway binary vectors (R4pGWB) enabling high-throughput promoter swapping for plant research
Development of r4 Gateway binary vectors (R4pGWB) enabling high-throughput promoter swapping for plant research
复制标题
DOI：
10.1271/bbb.70678
复制
发表时间：
2008-02-01
影响因子：
1.6
通讯作者：
Ishiguro, Sumie
中科院分区：
文献类型：
--
作者：
Nakagawa, Tsuyoshi;Nakamura, Shinya;Ishiguro, Sumie
我们开发了一系列新的Gateway双元载体R4pGWB，它们是设计用于在任何启动子和任何cDNA之间一步构建嵌合基因的植物转化载体。R4pGWB的结构几乎与无启动子类型的改进的pGWB（ImpGWB）相同，除了attR1位点被attR4取代，这使得这些载体能够与启动子和eDNA进入克隆进行三方重组。ImpGWB适合于高等植物中cDNA的启动子分析和组成型表达，而R4pGWB在所需启动子调控下表达cDNA方面具有很大的优势。
We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA. The structure of R4pGWBs is almost the same as the promoterless type of improved pGWBs (ImpGWBs), except that the attR1 site is replaced with attR4, which enables tripartite recombination of these vectors with promoter- and eDNA-entry clones. While ImpGWBs are suitable for promoter analysis and constitutive expression of cDNAs in higher plants, R4pGWBs have a great advantage in expressing a cDNA under the regulation of desired promoters.