Nicotine metabolism pathway in bacteria: mechanism, modification, and application
Nicotine metabolism pathway in bacteria: mechanism, modification, and application
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DOI：
10.1007/s00253-022-11763-y
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发表时间：
2022-01
影响因子：
5
通讯作者：
Zeling Zhang;Xiaotong Mei;Ziliang He;Xiya Xie;Yang Yang-Yang;Chengyu Mei;Dong Xue;Tong Hu
中科院分区：
文献类型：
--
作者：
Zeling Zhang;Xiaotong Mei;Ziliang He;Xiya Xie;Yang Yang-Yang;Chengyu Mei;Dong Xue;Tong Hu
尼古丁是一种有害的污染物，主要来自烟草工厂的废物。有必要通过生物修复等高效策略去除尼古丁。到目前为止，已经分离出越来越多的尼古丁降解菌株。但它们的降解效率和对高含量尼古丁的耐受性还不够高，不能应用于真实的环境中。因此，尼古丁代谢途径的修饰是有义务的，并且需要对尼古丁降解菌株的全细胞代谢进行全面的分子洞察。多组学技术的发展为微生物降解尼古丁的机理研究提供了新的思路和方法。到目前为止，尼古丁降解的三条途径，吡啶途径，吡咯烷途径，以及吡啶和吡咯烷途径的变体（VPP途径），已被明确确定在细菌中。多组学分析进一步揭示了不同菌株的基因组结构、调控机制以及三条途径的特异基因或酶。特别是多组学分析表明，功能模块共存于不同的基因组位点，并在提高细菌的降解效率方面发挥额外的作用。基于上述发现，基因组编辑策略变得更加可行，可以大大提高细菌对尼古丁的降解效率。
Nicotine is a harmful pollutant mainly from the waste of tobacco factories. It is necessary to remove nicotine via high efficient strategies such as bioremediation. So far, an increasing number of nicotine degrading strains have been isolated. However, their degrading efficiency and tolerance to high content nicotine is still not high enough for application in real environment. Thus, the modification of nicotine metabolism pathway is obligated and requires comprehensive molecular insights into whole cell metabolism of nicotine degrading strains. Obviously, the development of multi-omics technology has accelerated the mechanism study on microbial degradation of nicotine and supplied more novel strategy of strains modification. So far, three pathways of nicotine degradation, pyridine pathway, pyrrolidine pathway, and the variant of pyridine and pyrrolidine pathway (VPP pathway), have been clearly identified in bacteria. Muti-omics analysis further revealed specific genome architecture, regulation mechanism, and specific genes or enzymes of three pathways, in different strains. Especially, muti-omics analysis revealed that functional modules coexisted in different genome loci and played additional roles on enhanced degradation efficiency in bacteria. Based on the above discovery, genomic editing strategy becomes more feasible to greatly improve bacterial degrading efficiency of nicotine.