Detection of chondroitin sulfates and decorin in developing fetal and neonatal rat lung.
Detection of chondroitin sulfates and decorin in developing fetal and neonatal rat lung.
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检测发育中的胎儿和新生大鼠肺中的硫酸软骨素和核心蛋白聚糖。
DOI：
10.1152/ajplung.00160.2001
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Sannes,PhilipL
中科院分区：
文献类型：
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作者：
Wang,Yiqiong;Sakamoto,Kaori;Khosla,Jody;Sannes,PhilipL
硫酸软骨素及其相关蛋白聚糖是细胞外基质的组分，其作为发育中的肺的生长和分化特征的关键决定因素。在进行性器官成熟过程中其免疫组织化学分布的变化进行了检查与硫酸软骨素，非基底膜硫酸软骨素蛋白聚糖，和特定的硫酸软骨素蛋白聚糖核心蛋白聚糖在整个胎儿和新生儿和成年大鼠肺的单特异性抗体。肺泡和气道细胞外基质免疫染色严重的产前大鼠硫酸软骨素和硫酸软骨素蛋白多糖，而核心蛋白聚糖仅限于发展中的气道和血管。这些位点在出生后1-10天内保留了它们各自与所有抗体的反应性水平，但此后在肺泡区域逐渐减少和集中。在发育早期观察到的重染色被解释为反映了硫酸软骨素、硫酸软骨素蛋白聚糖和核心蛋白聚糖在快速生长的组织中的显著和广泛分布，而在稍后的时间点观察到的减少的和更集中的反应性与细胞外基质的纤维状元件的定位的已知集中模式和更分化的状态相一致。
Chondroitin sulfates and their related proteoglycans are components of extracellular matrix that act as key determinants of growth and differentiation characteristics of developing lungs. Changes in their immunohistochemical distribution during progressive organ maturation were examined with monospecific antibodies to chondroitin sulfate, a nonbasement membrane chondroitin sulfate proteoglycan, and the specific chondroitin sulfate-containing proteoglycan decorin in whole fetuses and lungs from newborn and adult rats. Alveolar and airway extracellular matrix immunostained heavily in the prenatal rat for both chondroitin sulfate and chondroitin sulfate proteoglycan, whereas decorin was confined to developing airways and vessels. These sites retained their respective levels of reactivity with all antibodies through 1–10 days postnatal but thereafter became progressively more diminished and focal in alveolar regions. The heavy staining seen early in development was interpreted to reflect a significant and wide distribution of chondroitin sulfates, chondroitin sulfate proteoglycans, and decorin in rapidly growing tissues, whereas the reduced and more focal reactivity observed at later time points coincided with known focal patterns of localization of fibrillar elements of the extracellular matrix and a more differentiated state.