Expression of members of the thrombospondin family by human skeletal tissues and cultured cells
Expression of members of the thrombospondin family by human skeletal tissues and cultured cells
复制标题
DOI：
10.1006/bbrc.1999.1380
复制
发表时间：
1999-09-24
影响因子：
3.1
通讯作者：
Gallagher, JA
中科院分区：
文献类型：
--
作者：
Carron, JA;Bowler, WB;Gallagher, JA
我们之前已经表明，多功能血小板糖蛋白血小板反应蛋白-1 （TSP-1）促进体外吸收测定。然而，TSP-1是多功能TSP分子家族中的一员，目前的研究是探讨TSP-1或其他TSP家族成员是否可能参与体内吸收调节。使用每种TSP家族成员特异性引物对培养的人骨细胞、培养的人软骨细胞和三个独立的人破骨细胞瘤组织样本进行RT-PCR。几乎在所有样本中都检测到TSP-2的mRNA，并且在上述组织的所有破骨细胞瘤中都检测到，而TSP-1的检测频率较低，仅在三种破骨细胞瘤中的一种中检测到，TSP-3， -4和COMP仅在少数病例中检测到。这些结果表明，TSP-2是骨骼组织中最常见的TSP家族成员，TSP-2而不是TSP-1可能是促进体内吸收的分子。(C) 1999学术出版社。
We have previously shown that the multifunctional platelet glycoprotein thrombospondin-1 (TSP-1) promotes resorption in an in vitro resorption assay. However, TSP-1 is one of a family of multifunctional TSP molecules, and the current study was undertaken to investigate whether it is TSP-1 or another TSP family member which may be involved in regulation of resorption in vivo. RT-PCR was performed on cultured human bone cells, cultured human chondrocytes, and three separate samples of human osteoclastoma tissue using primers specific for each TSP family member. mRNA for TSP-2 was detected in almost all samples, and significantly in all osteoclastomas in the above tissues, while TSP-1 was detected less frequently and was only seen in one of three osteoclastomas, TSP-3, -4, and COMP were detected only in a minority of cases. These results indicate that TSP-2 is the most common TSP family member found in skeletal tissues and that TSP-2, rather than TSP-1, may be the molecule responsible for promoting resorption in vivo. (C) 1999 Academic Press.