Change of the higher order structure of DNA induced by the complexation with intercalating synthetic polymer, as is visualized by fluorescence microscopy
Change of the higher order structure of DNA induced by the complexation with intercalating synthetic polymer, as is visualized by fluorescence microscopy
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DOI：
10.1093/nar/21.1.37
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发表时间：
1993-01
影响因子：
14.9
通讯作者：
K. Minagawa;Y. Matsuzawa;K. Yoshikawa;Yuichi Masubuchi;M. Matsumoto;M. Doi;C. Nishimura;M. Maeda
中科院分区：
文献类型：
--
作者：
K. Minagawa;Y. Matsuzawa;K. Yoshikawa;Yuichi Masubuchi;M. Matsumoto;M. Doi;C. Nishimura;M. Maeda
用荧光显微镜观察了T4 DNA、λ DNA及其与聚丙烯酰胺（PAAm）通过5-[（4-丙烯酰氨基）苯基]-3-氨基-7-（二甲氨基）-2-甲基吩嗪氯化物（5-[（4-acryloamino）phenyl]-3-amino-7-（dimethylamino）-2-methylphenazinium chloride）嵌入单元形成的复合物的电泳运动和布朗运动。在凝胶电泳中发现，T4 DNA/PAAm复合物的迁移速度比单独的T4 DNA慢，尽管它们在迁移过程中表现出相似的构象变化。λ DNA及其复合物在溶液中的平移扩散的定量分析表明，DNA分子由于PAAm的插入结合而延伸，表明复合物的假接枝结构。
The electrophoretic movement and Brownian motion of T4DNA, lambda DNA and their complexes with polyacrylamide (PAAm) via intercalative unit from 5-[(4-acryloylamino)phenyl]-3-amino-7-(dimethylamino)-2-methylp henazinium chloride were observed using fluorescence microscopy. It was found that T4DNA/PAAm complex migrates slower than T4DNA alone in gel electrophoresis, although they exhibit similar conformational change during the migration. Quantitative analyses of the translational diffusion of the lambda DNA and its complex in solution demonstrate that the DNA molecules extend due to intercalative binding of PAAm, suggesting the pseudo-grafting structure of the complex.