Contiguous Four‐guanosine Sequence in c‐myc Antisense Phosphorothioate Oligonucleotides Inhibits Cell Growth on Human Lung Cancer Cells: Possible Involvement of Cell Adhesion Inhibition
Contiguous Four‐guanosine Sequence in c‐myc Antisense Phosphorothioate Oligonucleotides Inhibits Cell Growth on Human Lung Cancer Cells: Possible Involvement of Cell Adhesion Inhibition
复制标题
c-myc 反义硫代磷酸寡核苷酸中的连续四鸟苷序列抑制人肺癌细胞的细胞生长：可能涉及细胞粘附抑制
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
T. Nukiwa
中科院分区：
文献类型：
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作者：
Y. Saijo;B. Uchiyama;T. Abe;K. Satoh;T. Nukiwa
A contiguous four‐guanosine (4G) sequence in c‐myc antisense phosphorothioate oligonucleotides caused an antiproliferative effect in smooth muscle cells. To investigate the antiproliferative effect of c‐myc antisense oligonucleotides on human lung cancer cell lines, we synthesized oligonucleotides of various lengths and sequences, focusing on the contiguous four‐guanosine (4G) sequence. While a c‐myc antisense oligonucleotide (20AS1 (4G)) targeted to the translation initiation codon of c‐myc mRNA inhibited cell growth of A549 cells by 69% at 10 μM, a scrambled oligonucleotide (20SCR1 (4G)) containing the contiguous four‐guanosine (4G) sequence also inhibited cell growth by 72% at the same dose. Although treatment with either 20AS1 (4G) or 20SCR1 (4G) inhibited cell adhesion by 70% at 10 μM, expression of c‐myc protein was significantly suppressed only by 20AS1 (4G) (62%), and was only weakly inhibited by 20SCR1 (4G) (32%). Furthermore, a small cell lung carcinoma cell line, Lu65, which can grow in suspension form, was highly resistant to 20AS1 (4G) treatment (IC50>20 μM). These results suggest that the cell growth inhibition by C‐myc antisense oligonucleotides containing the contiguous four‐guanosine (4G) sequence was possibly correlated with inhibition of cell adhesion, but not with inhibition of c‐myc protein expression, via a sequence‐specific non‐antisense mechanism.
抑制 c-myc 是糖皮质激素诱导的人白血病细胞裂解的关键步骤。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Thulasi,R;Harbour,DV;Thompson,EB
通讯作者：
Thompson,EB
DOI：
10.1016/s0021-9258(18)98398-5
发表时间：
1993-05
期刊：
The Journal of biological chemistry
影响因子：
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作者：
M. Iwamoto;K. Yagami;P. Lu Valle;B. Olsen;C. Petropoulos;D. Ewert;M. Pacifici
通讯作者：
M. Iwamoto;K. Yagami;P. Lu Valle;B. Olsen;C. Petropoulos;D. Ewert;M. Pacifici
硫代寡核苷酸序列对培养的人乳腺上皮细胞的影响。
DOI：
10.1089/ard.1993.3.67
发表时间：
1993
期刊：
Antisense research and development
影响因子：
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作者：
Yaswen,P;Stampfer,MR;Ghosh,K;Cohen,JS
通讯作者：
Cohen,JS