Evolutionary conservation of both the hydrophilic and hydrophobic nature of transmembrane residues.
Evolutionary conservation of both the hydrophilic and hydrophobic nature of transmembrane residues.
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DOI：
10.1006/jtbi.1995.0021
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发表时间：
1995-02
影响因子：
2
通讯作者：
R. Riek;M. Handschumacher;S. Sung;M. Tan;M. J. Glynias;M. Schluchter;J. Novotný;R. Graham
中科院分区：
文献类型：
--
作者：
R. Riek;M. Handschumacher;S. Sung;M. Tan;M. J. Glynias;M. Schluchter;J. Novotný;R. Graham
一种算法（HRG），开发允许成对比较的几个成员的大基因家族的多面体整合膜蛋白的对齐残基。使用疏水性尺度，该算法的应用允许细菌视紫红质，其结构已部分确定的多位蛋白质的跨膜结构域的数量和大小，以预测具有高度的准确性（灵敏度94%，特异性82%，用于预测膜嵌入或膜外位置的残基）。与之前报道的结构预测算法相反，通过应用HRG算法，从连接环中描绘推定的跨膜片段也更加明显，即使是来自不同物种的蛋白质。这表明强大的进化压力保守的疏水性和亲水性的残基在膜嵌入区域的多位蛋白质，如G-蛋白偶联受体超家族。这些和其他结构和功能的影响，显然从应用程序的HRG算法被认为是。
An algorithm (HRG), developed to allow the pairwise comparisons of the aligned residues of several members of large gene families of polytopic integral membrane proteins is described. Using hydrophobicity scales, application of this algorithm allows the number and size of the membrane-spanning domains of bacteriorhodopsin, a polytopic protein whose structure has been partially determined, to be predicted with a high degree of accuracy (sensitivity 94%, specificity 82% for predicting the membrane embedded or extramembranous location of residues). As opposed to previously reported structure-prediction algorithms, delineation of putative transmembrane segments from connecting loops is also more clearly evident with the application of the HRG algorithm, even with proteins from widely divergent species. This indicates strong evolutionary pressure for the conservation of both the hydrophobic and hydrophilic character of residues in membrane-embedded regions of polytopic proteins, such as those of the G-protein-coupled receptor superfamily. These and other structural and functional implications evident from the application of the HRG algorithm are considered.