DISSECTING THE HEMATOPOIETIC MICROENVIRONMENT. I. STEM CELL LODGMENT AND COMMITMENT, AND THE PROLIFERATION AND DIFFERENTIATION OF ERYTHROPOIETIC DESCENDANTS IN THE Sl/Sld MOUSE
DISSECTING THE HEMATOPOIETIC MICROENVIRONMENT. I. STEM CELL LODGMENT AND COMMITMENT, AND THE PROLIFERATION AND DIFFERENTIATION OF ERYTHROPOIETIC DESCENDANTS IN THE Sl/Sld MOUSE
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剖析造血微环境。
DOI：
10.1111/j.1365-2184.1974.tb00402.x
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发表时间：
1974
影响因子：
8.5
通讯作者：
N. Wolf
中科院分区：
文献类型：
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作者：
N. Wolf
本实验以贫血Sl/SLD小鼠及其正常(+/+)同基因对照小鼠为研究对象，探讨了骨髓基质调控红系形成的四个阶段：CFU着床、多能干细胞对红系的承诺、干细胞及其子代的增殖和子代向更成熟的分化。Sl/SLD小鼠的寄宿与正常小鼠相同，但承诺，尽管与骨髓相比，脾的特征不同，但低于正常。以脾集落大小和细胞类型含量衡量的增殖刺激作用甚至更弱。提示分化为更成熟的细胞的直接控制可能不受基质的控制。
The anemic Sl/Sld mouse and its normal (+/+) congenic control were used to explore the possibility of stromal control over four phases of erythropoiesis: CFU lodgment, commitment of multipotent stem cells to the erythropoietic line, proliferation of stem cells and their descendants, and the differentiation of those descendants into successively more mature forms. Lodgment was found to be the same in the Sl/Sld as in the normal mouse, but commitment, although characteristically different for spleen compared to the bone marrow, was subnormal. The stimulus to proliferate, as measured by spleen colony size and cell type content, was even more reduced. It is suggested that the direct control of differentiation into more mature cells may not be under stromal control.